caa a22 4500 386322597 CHVBK 20180307111632.0 cr unu---uuuuu 161130e198809 xx s 000 0 eng 10.1079/BJN19880100 doi S0007114588001011 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880100 Effect of tannin-rich leaves of oak (Quercus incana) on various microbial enzyme activities of the bovine rumen [Elektronische Daten] 1. The objective of the present experiment was to study the effects of oak (Quercus incana) leaves rich in tannins on various enzyme activities of the bovine rumen. 2. The procedure employed was incubation of tannin-rich, very-low-tannin or virtually tannin-free leaves in nylon-gauze bags in the rumen, and determination of enzyme activities in microbes tightly bound to the solid matrix and in microbes loosely plus tightly attached to the solid matrix. 3. The activities of urease (EC 3.5.1.5), carboxymethylcellulase, glutamate dehydrogenase (EC 1.4.1.2) and alanine aminotransferase (glutamic-pyruvic transaminase) (EC 2.6.1.2) were significantly lower in the tannin-rich group, whereas the activities of glutamate ammonia ligase (glutamine synthetase) (EC 6.3.1.2; both yγ- glutamyltransferase (EC 2.3.2.2) and the forward reaction) were higher in the tannin-rich group. These changes were more marked in micro-organisms tightly bound to the solid matrix than in the more complex microbial compartment. 4. The protein, DNA and RNA contents, and protein: RNA ratio, were significantly lower in the tannin-rich group, whereas no difference was observed for protein: DNA between the groups. 5. Effects of tannin-containing extracts of oak leaves on various rumen enzymes in vitro showed a trend similar to that observed in nylon-gauze bags, suggesting that the changes observed in various compartments were due to the tannins of oak leaves. Copyright © The Nutrition Society 1988 Makkar H. P. S. Indian Veterinary Research Institute, Regional Station, Palampur Kangra Valley 176 061, Himachal Pradesh, India Singh B. Indian Veterinary Research Institute, Regional Station, Palampur Kangra Valley 176 061, Himachal Pradesh, India Dawra R. K. Indian Veterinary Research Institute, Regional Station, Palampur Kangra Valley 176 061, Himachal Pradesh, India British Journal of Nutrition Cambridge University Press 60/2(1988-09), 287-296 0007-1145 60:2<287 1988 60 BJN https://doi.org/10.1079/BJN19880100 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880100 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Makkar H. P. S. Indian Veterinary Research Institute, Regional Station, Palampur Kangra Valley 176 061, Himachal Pradesh, India NATIONALLICENCE 700 1- Singh B. Indian Veterinary Research Institute, Regional Station, Palampur Kangra Valley 176 061, Himachal Pradesh, India NATIONALLICENCE 700 1- Dawra R. K. Indian Veterinary Research Institute, Regional Station, Palampur Kangra Valley 176 061, Himachal Pradesh, India NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 60/2(1988-09), 287-296 0007-1145 60:2<287 1988 60 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge