caa a22 4500 386322872 CHVBK 20180307111633.0 cr unu---uuuuu 161130e198811 xx s 000 0 eng 10.1079/BJN19880117 doi S0007114588001199 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880117 Nitrogen balance study in young Nigerian adult males using four levels of protein intake [Elektronische Daten] 1. The present study was carried out to estimate precisely, via the nitrogen balance technique, the protein requirement of Nigerians (earlier estimated via the obligatory N method) using graded levels of protein intake. 2. Fifteen medical students of the University of Ibadan who volunteered to participate in the study were given graded levels of protein (0.3, 0.45, 0.6 and 0.75 g/kg body-weight per d) derived from foods similar to those usually consumed by the subjects. 3. Each subject was given each of the dietary protein levels for a period of 10 d. Subjects were divided into two groups and the feeding pattern followed a criss-cross design with one group starting with the highest level of protein intake (0.75 g) and the second group starting with the lowest level of protein intake (0.3 g). Mean energy intake during each of the eleven experimental periods was maintained at 0.2 MJ/kg per d. After an initial 5d adaptation period in each experimental period, 24 h urine and faecal samples were collected in marked containers for five consecutive days for N determination. 4. Mean N balance during consumption of the four protein levels (0.30, 0.45, 0.6 and 0.75 g/kg) were −11.02 (SD 8.07), −9.90 (SD 6.64), + 9.70 (SD 4.15) and + 5.13 (SD 4.62) respectively. Using regression analysis, the mean daily N requirement was estimated at 110.25 mg N/kg body-weight (0.69 g protein/kg body-weight). Estimates of allowances for individual variations to cover 97.5% of the population adjusted this value to 0.75 g protein/kg body-weight. Net protein utilization for the diet at maintenance level was estimated at 57.5. Copyright © The Nutrition Society 1988 Atinmo T. Department of Human Nutrition, College of Medicine, University of Ibadan, Ibadan, Nigeria Mbofung C. M. F. Department of Human Nutrition, College of Medicine, University of Ibadan, Ibadan, Nigeria Egun G. Department of Human Nutrition, College of Medicine, University of Ibadan, Ibadan, Nigeria Osotimehin B. Department of Pathology, College of Medicine, University of Ibadan, Ibadan, Nigeria British Journal of Nutrition Cambridge University Press 60/3(1988-11), 451-458 0007-1145 60:3<451 1988 60 BJN https://doi.org/10.1079/BJN19880117 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880117 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Atinmo T. Department of Human Nutrition, College of Medicine, University of Ibadan, Ibadan, Nigeria NATIONALLICENCE 700 1- Mbofung C. M. F. Department of Human Nutrition, College of Medicine, University of Ibadan, Ibadan, Nigeria NATIONALLICENCE 700 1- Egun G. Department of Human Nutrition, College of Medicine, University of Ibadan, Ibadan, Nigeria NATIONALLICENCE 700 1- Osotimehin B. Department of Pathology, College of Medicine, University of Ibadan, Ibadan, Nigeria NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 60/3(1988-11), 451-458 0007-1145 60:3<451 1988 60 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge