caa a22 4500 386322902 CHVBK 20180307111633.0 cr unu---uuuuu 161130e198811 xx s 000 0 eng 10.1079/BJN19880132 doi S0007114588001345 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880132 Effects of age, and protein malnutrition followed by a balanced diet on the non-parallel change in digestive enzymes in the pancreas and their secretion in the rat [Elektronische Daten] 1. Ninety male Wistar rats were divided into two groups. A control group (C) was fed on a balanced diet, containing 200 g protein/kg for 51 d. An experimental group (E) was fed on a low-protein diet containing 50 g protein/kg for 28 d (PM), and then on a balanced diet for 23 d (BR). At different days of PM and BR, the pancreas and the pancreatic juice were collected 40 min after injection of 0.1 mCi [3H]leucine. The amounts of amylase (EC 3.2.1.1), trypsinogen 2 (EC 3.4.21.4), chymotrypsinogen 1 (EC 3.4.21.1) and lipase (EC 3.1.1.3) were determined after separation by the isoelectric focussing technique. Incorporation of [3H]leucine into the four hydrolases of pancreatic juice and pancreas was also determined. 2. In control rats a progressive increase in the concentration of digestive enzymes and the amounts secreted were observed with age. Maturation was reached when the rats were 9 weeks old. In rats E, PM inhibited maturation of the pancreas. However, individual enzymes were not affected to the same extent and at the same time. As soon as re-feeding was initiated, pancreas maturation took place and a significant increase in these variables was observed. The increases varied according to the hydrolase and did not appear at the same time. 3. In control rats, a preferential secretion of newly synthesized enzymes was observed in young rats, whereas with age, the proportion of newly synthesized enzymes excreted decreased slowly. In group E rats, at the beginning of PM, the proportion of newly synthesized enzymes secreted was very low and increased with time. 4. In rats C and E, our results indicated a non-parallelism between pancreatic enzyme levels and amounts secreted. This non-parallelism was different in both groups, it was changed with age and pancreas maturation in group C, and according to nutritional state in group E. Copyright © The Nutrition Society 1988 Prost Josiane Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 - 21004 Dijon Cedex, France Belleville Jacques Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 - 21004 Dijon Cedex, France Valantin-Rollet Carole Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 - 21004 Dijon Cedex, France British Journal of Nutrition Cambridge University Press 60/3(1988-11), 619-631 0007-1145 60:3<619 1988 60 BJN https://doi.org/10.1079/BJN19880132 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880132 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Prost Josiane Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 - 21004 Dijon Cedex, France NATIONALLICENCE 700 1- Belleville Jacques Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 - 21004 Dijon Cedex, France NATIONALLICENCE 700 1- Valantin-Rollet Carole Université de Bourgogne, Laboratoire de Physiologie de la Nutrition, Faculté des Sciences Mirande, BP 138 - 21004 Dijon Cedex, France NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 60/3(1988-11), 619-631 0007-1145 60:3<619 1988 60 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge