caa a22 4500 386322910 CHVBK 20180307111633.0 cr unu---uuuuu 161130e198811 xx s 000 0 eng 10.1079/BJN19880127 doi S0007114588001291 pii (NATIONALLICENCE)cambridge-10.1079/BJN19880127 Partition of portal-drained visceral net flux in beef steers [Elektronische Daten] 2. Net flux of volatile fatty acids, D-β-hydroxybutyrate and L-lactate across stomach and post-stomach tissues* 1. Net flux of volatile fatty acids (VFA), D-β-hyroxybutyrate (BOHB) and L-lactate across post-stomach (anterior mesenteric-drained viscera (MDV)), stomach and total hepatic portal-drained viscera (PDV) tissues was measured in two beef steers (mean live weight 390 kg). 2. Net flux was measured while steers were fed, in sequence, on (1) chopped lucerne (Medicato sativa) (twelve meals/d), (2) chopped lucerne (two meals/d) and (3) a pelleted concentrate diet containing 780 g ground maize/ kg (two meals/d). 3. Five hourly net flux measurements were obtained on 2 d for each dietary regimen, beginning 0.5 h before a meal delivered at 08.00 hours. Net flux was calculated as venous-arterial concentration differences (VA) multiplied by blood flow (measured by downstream dilution of p-aminohippurate (PAH)). 4. Stomach tissues accounted for 85 to over 100% of net VFA and BOHB appearance across PDV. Net appearance across stomach tissues represented 74% of net PDV L-lactate appearance. 5. When lucerne was given, there was net utilization of arterial acetate and BOHB across MDV. This MDV utilization may reflect acetate and BOHB use as an energy source or their incorporation into mesenteric fat. 6. When concentrate was given, more n-butyrate and n-valerate and less L-lactate appeared across PDV and less 3-methylbutyrate appeared across stomach tissues than when lucerne was given. Postprandial increases in VFA, BOHB and L-lactate net flux across PDV followed meal-feeding of lucerne. 7. On a net basis, the relative contribution of MDV tissues to total PDV net appearance of VFA and BOHB was small (< 15%) in these steers. Copyright © The Nutrition Society 1988 Reynolds Christopher K. U.S. Department of Agriculture, Agricultural Research Service, Ruminant Nutrition Laboratory, Building 162, BARC-East, Beltsville, MD 20705, USA and University of Maryland, College Park, MD 20742, USA Huntington Gerald B. U.S. Department of Agriculture, Agricultural Research Service, Ruminant Nutrition Laboratory, Building 162, BARC-East, Beltsville, MD 20705, USA and University of Maryland, College Park, MD 20742, USA British Journal of Nutrition Cambridge University Press 60/3(1988-11), 553-562 0007-1145 60:3<553 1988 60 BJN https://doi.org/10.1079/BJN19880127 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1079/BJN19880127 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Reynolds Christopher K. U.S. Department of Agriculture, Agricultural Research Service, Ruminant Nutrition Laboratory, Building 162, BARC-East, Beltsville, MD 20705, USA and University of Maryland, College Park, MD 20742, USA NATIONALLICENCE 700 1- Huntington Gerald B. U.S. Department of Agriculture, Agricultural Research Service, Ruminant Nutrition Laboratory, Building 162, BARC-East, Beltsville, MD 20705, USA and University of Maryland, College Park, MD 20742, USA NATIONALLICENCE 773 0- British Journal of Nutrition Cambridge University Press 60/3(1988-11), 553-562 0007-1145 60:3<553 1988 60 BJN CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge