caa a22 4500 38634714X CHVBK 20180307111815.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S002221510011117X doi S002221510011117X pii (NATIONALLICENCE)cambridge-10.1017/S002221510011117X Effect of acoustic overstimulation on the glycocalyx and the ciliary interconnections in the organ of Corti: High resolution scanning electron microscopic investigation [Elektronische Daten] Changes in ciliary interconnections in the organ of Corti are described after acoustic overstimulation using a special high resolution scanning electron microscope and tannic acid-osmium staining technique, giving an almost three dimensional view. Guinea pigs were exposed to a 3.85 kHz pure tone at an intensity of 120 dB for 22.5 minutes. The first detectable change was a disarrangement of the cilia with a loosening of the interconnections. The ciliary plasma membrane presented with an abnormally smooth appearance. The tip links connecting the tips of the stereocilia to their taller neighbours were also affected showing elongation or even disappearance. The fine granules which cover the tips of the tallest stereocilia of the outer hair cells were decreased. These findings suggest that acoustic overstimulation may affect the carbohydrate metabolism exceding to degeneration of ciliary interconnections resulting in a disarrangement and detachment of cilia. The tip links, which may participate in sensory cell transduction, seem also to be affected by acoustic overstimulation. Copyright © JLO (1984) Limited 1989 Takumida M. Department of Otolaryngology, Hiroshima University School of Medicine, Hiroshima, Japan Fredelius L. Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden Bagger-Sjöbäck D. Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden Harada Y. Department of Otolaryngology, Hiroshima University School of Medicine, Hiroshima, Japan Wersäll J. Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden The Journal of Laryngology & Otology Cambridge University Press 103/12(1989-12), 1125-1129 0022-2151 103:12<1125 1989 103 JLO https://doi.org/10.1017/S002221510011117X text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S002221510011117X text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takumida M. Department of Otolaryngology, Hiroshima University School of Medicine, Hiroshima, Japan NATIONALLICENCE 700 1- Fredelius L. Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden NATIONALLICENCE 700 1- Bagger-Sjöbäck D. Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden NATIONALLICENCE 700 1- Harada Y. Department of Otolaryngology, Hiroshima University School of Medicine, Hiroshima, Japan NATIONALLICENCE 700 1- Wersäll J. Department of Otolaryngology, Karolinska Hospital, Karolinska Institue, Stockholm, Sweden NATIONALLICENCE 773 0- The Journal of Laryngology & Otology Cambridge University Press 103/12(1989-12), 1125-1129 0022-2151 103:12<1125 1989 103 JLO CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge