caa a22 4500 38637581X CHVBK 20180307112017.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S1742758400003489 doi S1742758400003489 pii (NATIONALLICENCE)cambridge-10.1017/S1742758400003489 Purification and characterization of vitellin from the palo verde beetle, Derobracus geminatus [Elektronische Daten] Isolation of vitellin from mature eggs of the palo verde beetle, Derobracus geminatus, was achieved by a combination of gel permeation and anion-exchange chromatography. It is a phosphoglycolipoprotein (Mr ˜250,000) that contains 8% lipids, 4% carbohydrates and 0.2% protein-bound phosphorus. The vitellin is composed of three apoproteins designated I (Mr ˜160,000), II (Mr ˜140,000) and III (Mr ˜50,000). The carbohydrate moiety is composed exclusively of mannose and N-acetylglucosamine. All three apoproteins are glycosylated as shown by flourescein isothiocyanate-conjugated concanavalin A and periodate-Schiff (PAS) staining suggesting the presence of a high mannose structure. Further evidence for the high mannose structure was obtained by the ability of endo-β-N-acetylglucosaminidase H treatment to remove the carbohydrate moieties on the apoproteins. Copyright © ICIPE 1989 Derobracus geminatus nationallicence vitellin nationallicence physical-chemical studies nationallicence Derobracus geminatus nationallicence vitellus nationallicence etudes physicochimiques nationallicence Osir Ellie O. Department of Biochemistry, Biological Sciences West, University of Arizona Tucson, AZ 85721, USA Law John H. Department of Biochemistry, Biological Sciences West, University of Arizona Tucson, AZ 85721, USA International Journal of Tropical Insect Science Cambridge University Press 10/3(1989-06), 259-267 1742-7584 10:3<259 1989 10 JTI https://doi.org/10.1017/S1742758400003489 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S1742758400003489 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Osir Ellie O. Department of Biochemistry, Biological Sciences West, University of Arizona Tucson, AZ 85721, USA NATIONALLICENCE 700 1- Law John H. Department of Biochemistry, Biological Sciences West, University of Arizona Tucson, AZ 85721, USA NATIONALLICENCE 773 0- International Journal of Tropical Insect Science Cambridge University Press 10/3(1989-06), 259-267 1742-7584 10:3<259 1989 10 JTI CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge