caa a22 4500 386375844 CHVBK 20180307112018.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S1742758400003532 doi S1742758400003532 pii (NATIONALLICENCE)cambridge-10.1017/S1742758400003532 Taste sensilla responses to limonoids, natural insect antifeedants [Elektronische Daten] Limonoids such as deoxylimonin, obacunone and pedoniti are known to have antifeedant effects against some lepidopteran pests. However, there is no electrophysiological data showing the effect these compounds have on taste receptor cells. This information gap is due to the fact that limonoids are insoluble in water and this makes it difficult to apply the tip-recording technique in an electrophysiological bioassay of limonoids. This problem was solved by dissolving the limonoids in a mixture of 50 % tetrahydrof uran and 50 % aqueous NaCl. Limonoids and sucrose so dissolved were used to investigate responses of Eldana saccharina maxillary styloconic sensilla. The solvent mixture did not appear to damage the taste receptor cells and the taste receptor responses to solutes dissolved in that solvent were reproducible. Sucrose stimulated a cell in the medial as well as in the lateral styloconic sensilla. Deoxylimonin alone stimulated a cell in the medial sensillum but not in the lateral sensillum. When presented with sucrose, deoxylimonin inhibited the sugar receptor cell and this was also true for obacunone. Copyright © ICIPE 1989 Limonoids nationallicence tetrahydrofuran nationallicence antifeedant nationallicence Eldana saccharina nationallicence taste sensilla nationallicence Lepidoptera nationallicence Limonoides nationallicence tétrahydrofurane nationallicence anti-appétant nationallicence Eldana saccharina nationallicence sensilles gustatifs nationallicence lepidoptera nationallicence Waladde S. M. The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya Hassanali A. The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya Ochieng S. A. The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya International Journal of Tropical Insect Science Cambridge University Press 10/3(1989-06), 301-308 1742-7584 10:3<301 1989 10 JTI https://doi.org/10.1017/S1742758400003532 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S1742758400003532 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Waladde S. M. The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya NATIONALLICENCE 700 1- Hassanali A. The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya NATIONALLICENCE 700 1- Ochieng S. A. The International Centre of Insect Physiology and Ecology (ICIPE), P. O. Box 30772, Nairobi, Kenya NATIONALLICENCE 773 0- International Journal of Tropical Insect Science Cambridge University Press 10/3(1989-06), 301-308 1742-7584 10:3<301 1989 10 JTI CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge