caa a22 4500 386388008 CHVBK 20180307112105.0 cr unu---uuuuu 161130e198909 xx s 000 0 eng 10.1017/S0022149X00009019 doi S0022149X00009019 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X00009019 Fate of developing larvae of Onchocerca lienalis and O.volvulus in micropore chambers implanted into laboratory hosts [Elektronische Daten] Infective larvae of Onchocerca lienalis and O.volvulus implanted subcutaneously within micropore chambers into laboratory hosts moulted to the fourth stage (L4) and underwent limited development and growth. Similar recoveries of O. lienalis L4 larvae in the range of 33-66% were obtained from chambers implanted into CBA and BALB/c strains of mice, jirds, and the natural bovine host. A relatively constant proportion of larvae survived up to 24 days post implantation and thereafter recoveries declined, although some worms were still alive after 96 days. Recoveries of O. volvulus L4 larvae from chambers given to normal or T-cell deprived mice were equivalent to one another and to those obtained with O. lienalis. Moulting of O. lienalis in chambers was observed on days 3 and 5, in close accordance with the timing of the third moult in cattle following systemic infection. Moulting of O. volvulus occurred between days 3-6. Morphological changes in developing larvae included a small but significant increase in length, a transient increase in width, and early development of the spicular primordia and genital tube. L4 larvae of O. lienalis, but not those of O. volvulus, exhibited 3 distinct caudal papillae not present on infective larvae. Copyright © Cambridge University Press 1989 Onchocerca lienalis nationallicence Onchocerca volvulus nationallicence mouse nationallicence Meriones unguiculatus nationallicence subcutaneous implantation nationallicence development nationallicence Bianco A. E. Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK Mustafa M. B. Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK Ham P. J. Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK Journal of Helminthology Cambridge University Press 63/3(1989-09), 218-226 0022-149X 63:3<218 1989 63 JHL https://doi.org/10.1017/S0022149X00009019 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X00009019 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bianco A. E. Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK NATIONALLICENCE 700 1- Mustafa M. B. Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK NATIONALLICENCE 700 1- Ham P. J. Department of Medical Helminthology, London School of Hygiene and Tropical Medicine, 395 Hatfield Road, St Albans, Herts AL4 0XQ, UK NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 63/3(1989-09), 218-226 0022-149X 63:3<218 1989 63 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge