caa a22 4500 386388091 CHVBK 20180307112105.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S0022149X00009160 doi S0022149X00009160 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X00009160 Lipid metabolism in Trichuris globulosa (Nematoda) [Elektronische Daten] Adult males and females of Trichuris globulosa, an intestinal nematode parasite of goats, were studied for their lipid composition, capability of incorporation of (Na)-1-14C-acetate into different lipid classes and the activity of certain key enzymes of lipid metabolism. The parasite possesses a large variety of lipids including certain complex lipids. These are phosphatidylcholine (PC), diphosphatidylglycerol (cardiolipin), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), phosphatidylserine (PS), phosphatidylinositol (PI), plasmalogens (choline+ethanolamine), mono-, di- and triacylglycerols, free and esterified cholesterol, non-esterified fatty acids (NEFA), gangliosides, cerebrosides (glycosyl ceramide) and sulphuric acid esters of cerebrosides (sulphatides). The females contain more lipids than males, particularly the acylglycerols and phospholipids, possibly to meet the energy requirement and structural entities for the daily production of large numbers of eggs. Incorporation studies of labelled substrate, sodium-1-14C acetate demonstrate that the adult female has extremely active mechanisms for biosynthesizing these lipids. Most of the labels are found in PC, PE, SM, acylglycerols, NEFA, gangliosides, cerebrosides and sulphatides. Cholesterol, although a minor component of the parasitic lipids, incorporates large amount of label and also undergoes fast turnover. Kinetic analysis of the incorporation by measuring the rate constant (k) and half life (t½) reveals that gangliosides are the fastest biosynthesizing and turning over lipids, although they constitute only 0·1% of the total lipids. The presence of important enzymes of lipid biosynthesis, glucose-6-phosphate dehydrogenase, malate dehydrogenase and hydroxymethyl glutaryl-CoA reductase and an enzyme of lipid ester hydrolysis, triacylglycerol lipase, is also established in T. globulosa. Michaelis-Menten kinetic characteristics of the parasitic enzymes (Km, Vmax, v and the first order rate constant, k) are comparable with those of rat liver homogenates. Copyright © Cambridge University Press 1989 Trichuris globulosa nationallicence lipid metabolism nationallicence 14C-acetate incorporation nationallicence Sarwal Rashmi Department of Zoology, Panjab University, Chandigarh-160014, India Sanyal S. N. Department of Zoology, Panjab University, Chandigarh-160014, India Khera S. Department of Zoology, Panjab University, Chandigarh-160014, India Journal of Helminthology Cambridge University Press 63/4(1989-12), 287-297 0022-149X 63:4<287 1989 63 JHL https://doi.org/10.1017/S0022149X00009160 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X00009160 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sarwal Rashmi Department of Zoology, Panjab University, Chandigarh-160014, India NATIONALLICENCE 700 1- Sanyal S. N. Department of Zoology, Panjab University, Chandigarh-160014, India NATIONALLICENCE 700 1- Khera S. Department of Zoology, Panjab University, Chandigarh-160014, India NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 63/4(1989-12), 287-297 0022-149X 63:4<287 1989 63 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge