caa a22 4500 38638813X CHVBK 20180307112105.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S0022149X00009159 doi S0022149X00009159 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X00009159 Prociv Paul Department of Parasitology, The University of Queensland, St Lucia, Queensland 4067, Australia Observations on the post-mortem migration of nematode larvae and its role in tissue digestion techniques [Elektronische Daten] [Paul Prociv] The importance of larval viability in relation to the success of recovery techniques and to the interpretation of histological and migration studies was tested using mice infected with Toxocara pteropodis, T. canis and T. cati. Little difference was found in total numbers of viable larvae recovered from tissues incubated for 18 hours in 0·85% saline, 2% trypsin or 1% pepsin, but the rates of larval egress did vary. The recovery of dead larvae by digestion techniques was much lower. Larvae migrated in tissues undergoing fixation, which influenced histological findings. Larvae of T. canis did not undergo significant peritoneal migration in acutely-infected mice, but did penetrate their intestinal walls in large numbers within 4 hours of death. The implications of these findings in experimental studies are discussed. Copyright © Cambridge University Press 1989 Toxocara pteropodis nationallicence Toxocara canis nationallicence Toxocara cati nationallicence Ascaris lumbricoides nationallicence digestion techniques nationallicence nematode larvae nationallicence larval migration nationallicence mous nationallicence Journal of Helminthology Cambridge University Press 63/4(1989-12), 281-286 0022-149X 63:4<281 1989 63 JHL https://doi.org/10.1017/S0022149X00009159 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X00009159 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Prociv Paul Department of Parasitology, The University of Queensland, St Lucia, Queensland 4067, Australia NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 63/4(1989-12), 281-286 0022-149X 63:4<281 1989 63 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge