caa a22 4500 386388202 CHVBK 20180307112105.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S0022149X00009214 doi S0022149X00009214 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X00009214 Studies on host specificity in Paragonimus westermani: II. Histochemical and cytochemical characterization of metacercariae and worms from rats and dogs [Elektronische Daten] Histochemical tests were done on newly excysted metacercariae and worms recovered from an abnormal host (rat) and the definitive host (dog) for some oxidoreductases, phosphatases and glycosidases. The results demonstrate that rat worms have enzymatic distribution and intensities more similar to those of metacercariae than to adult worms from dogs. Ultracytochemical examination of acid and alkaline phosphatase and Mg-ATPase activity was also carried out. Acid phosphatase activity occurred exceptionally in the excretory bladder and caeca of dog worms. No activity was observed in rat worms except for lysosomal granules in the tegument. Alkaline phosphatase activity was exhibited in the excretory bladder in both dog and rat worms. Mg-ATPase activity occurred in the tegument and parenchymal cells in dog worms and in the excretory bladder in rat worms. In metacercariae, little or no reaction for these enzymes was present except for Mg-ATPase activity on the excretory ducts. These observations, together with the histochemical results, indicate that metabolic activity in rat worms is higher than in metacercariae although it is strongly reduced compared with dog worms. Copyright © Cambridge University Press 1989 Paragonimus westermani nationallicence histochemistry nationallicence cytochemistry nationallicence host specificity nationallicence Fujino Takahiro Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan Fukuda Koichi Department of Parasitology, National Defence Medical College, Tokorozawa 359, Japan Hamajima Fusanori Department of Parasitology, National Defence Medical College, Tokorozawa 359, Japan Ishii Yoichi Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan Journal of Helminthology Cambridge University Press 63/4(1989-12), 315-327 0022-149X 63:4<315 1989 63 JHL https://doi.org/10.1017/S0022149X00009214 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X00009214 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Fujino Takahiro Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan NATIONALLICENCE 700 1- Fukuda Koichi Department of Parasitology, National Defence Medical College, Tokorozawa 359, Japan NATIONALLICENCE 700 1- Hamajima Fusanori Department of Parasitology, National Defence Medical College, Tokorozawa 359, Japan NATIONALLICENCE 700 1- Ishii Yoichi Department of Parasitology, Faculty of Medicine, Kyushu University, Fukuoka 812, Japan NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 63/4(1989-12), 315-327 0022-149X 63:4<315 1989 63 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge