caa a22 4500 386388504 CHVBK 20180307112106.0 cr unu---uuuuu 161130e198903 xx s 000 0 eng 10.1017/S0022149X00008646 doi S0022149X00008646 pii (NATIONALLICENCE)cambridge-10.1017/S0022149X00008646 Leukocyte accumulation in sparganosis: further characterization of an eosinophil chemotactic factor of the plerocercoid of Spirometra erinacei [Elektronische Daten] An eosinophil chemotactic factor (ECF) was partially purified from plerocercoids of Spirometra erinacei by a combination of anion-exchange chromatography on DE52 and gel filtration on Sephacryl S-200. The molecular weight of ECF was estimated to be 25 000-45 000 by high-pressure liquid chromatography. The ECF was bound with concanavalin A-Sepharose. The ECF was sensitive to periodate oxidation and to heating (56°C, 30 min). On isoelectric focusing, eosinophil chemotactic activity was clearly revealed at pI 4·1. These results suggest that ECF of S. erinacei plerocercoid is an acidic glycoprotein. An intradermal injection of ECF induced eosinophil attractions in the normal guinea pig skin. Copyright © Cambridge University Press 1989 Spirometra erinacei nationallicence eosinophil chemotactic factor nationallicence Horii Y. Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan Owhashi M. Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan Ishii A. Department of Parasitology, Okayama University Medical School, Shikata-cho, Okayama 700, Japan Fujita K. Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan Journal of Helminthology Cambridge University Press 63/1(1989-03), 6-12 0022-149X 63:1<6 1989 63 JHL https://doi.org/10.1017/S0022149X00008646 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0022149X00008646 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Horii Y. Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan NATIONALLICENCE 700 1- Owhashi M. Department of Parasitology, Miyazaki Medical College, Kiyotake, Miyazaki 889-16, Japan NATIONALLICENCE 700 1- Ishii A. Department of Parasitology, Okayama University Medical School, Shikata-cho, Okayama 700, Japan NATIONALLICENCE 700 1- Fujita K. Department of Medical Zoology, Nagasaki University School of Medicine, Sakamoto-machi, Nagasaki 852, Japan NATIONALLICENCE 773 0- Journal of Helminthology Cambridge University Press 63/1(1989-03), 6-12 0022-149X 63:1<6 1989 63 JHL CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge