caa a22 4500 386392641 CHVBK 20180307112124.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S0016672300028652 doi S0016672300028652 pii (NATIONALLICENCE)cambridge-10.1017/S0016672300028652 Restriction map and α-amylase activity variation among Drosophila mutation accumulation lines [Elektronische Daten] The specific activities of α-amylase were measured for two sets of mutation accumulation lines, each set having originated from a different lethal-carrying second chromosome and SM1(Cy) chromosome and having been maintained by a balanced lethal system for about 300 generations. Significant variation was found to have accumulated among lines of both sets. Because of dysgenic crosses in the early generations of mutation accumulation, insertions or deletions of transposable elements in the Amy gene region were suspected of being the cause of this variation. In order to test this possibility, the structural changes in the 14 kb region of these chromosomes that includes the structural genes for α-amylase were investigated by restriction map analysis. We found that most part of the activity variation is due to replacements of a chromosomal region of SM1(Cy), including the structural genes for α-amylase, by the corresponding regions of the lethal chromosomes. One line also contained an insertion in this region but this line has an intermediate activity value. Thus, insertions of transposable elements into the Amy gene region were not found to be responsible for the new variation observed in α-amylase activity. If we remove those lines with structural changes from the analysis, the genetic variance of α-amylase specific activity among lines becomes non-significant in both sets of chromosomes. Copyright © Cambridge University Press 1989 Tachida H. Department of Statistics, North Carolina State University, Box 8203, Raleigh, NC 27695-8203 Harada K. Department of Biology, Kyushu University 33, Fukuoka 812, Japan Langley C. H. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC27709 Aquadro C. F. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC27709 Yamazaki T. Department of Biology, Kyushu University 33, Fukuoka 812, Japan Cockerham C. C. Department of Statistics, North Carolina State University, Box 8203, Raleigh, NC 27695-8203 Mukai T. Department of Biology, Kyushu University 33, Fukuoka 812, Japan Genetical Research Cambridge University Press 54/3(1989-12), 197-203 0016-6723 54:3<197 1989 54 GRH https://doi.org/10.1017/S0016672300028652 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0016672300028652 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tachida H. Department of Statistics, North Carolina State University, Box 8203, Raleigh, NC 27695-8203 NATIONALLICENCE 700 1- Harada K. Department of Biology, Kyushu University 33, Fukuoka 812, Japan NATIONALLICENCE 700 1- Langley C. H. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC27709 NATIONALLICENCE 700 1- Aquadro C. F. Laboratory of Molecular Genetics, National Institute of Environmental Health Sciences, Research Triangle Park, NC27709 NATIONALLICENCE 700 1- Yamazaki T. Department of Biology, Kyushu University 33, Fukuoka 812, Japan NATIONALLICENCE 700 1- Cockerham C. C. Department of Statistics, North Carolina State University, Box 8203, Raleigh, NC 27695-8203 NATIONALLICENCE 700 1- Mukai T. Department of Biology, Kyushu University 33, Fukuoka 812, Japan NATIONALLICENCE 773 0- Genetical Research Cambridge University Press 54/3(1989-12), 197-203 0016-6723 54:3<197 1989 54 GRH CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge