caa a22 4500 386392854 CHVBK 20180307112125.0 cr unu---uuuuu 161130e198902 xx s 000 0 eng 10.1017/S0016672300027828 doi S0016672300027828 pii (NATIONALLICENCE)cambridge-10.1017/S0016672300027828 The in vivo and in vitro transmission frequencies of the tw5-haplotype in mice [Elektronische Daten] The recessive tω5-haplotype, a complete haplotype, is transmitted by heterozygous male mice at very high frequencies (> 0·90) in normal matings. The present studies were undertaken to determine the effects of delayed matings and in vitro fertilizations on this phenotypic expression. Males carrying the tω5-haplotype ( + / tω5) were first tested for their frequencies of transmission of the mutant 17th chromosome in both normal and delayed matings. Spermatozoa obtained from these same males were then used to fertilize eggs in vitro. The in vivo and in vitro transmission frequencies were found to be statistically equivalent in all types of inseminations. An in vitro fertilization time course study showed that the same percentages of eggs are fertilized by tω5- bearing spermatozoa when the gametes are coincubated for either 2 or 6 h. The data lead to the conclusion that the transmission frequency of the tω5-haplotype is not affected either by the length of time elapsing between insemination and fertilization or by the environment in which fertilization occurs. Copyright © Cambridge University Press 1989 Garside William Department of Biology, Temple University, Philadelphia, PA 19122 Hillman Nina Department of Biology, Temple University, Philadelphia, PA 19122 Genetical Research Cambridge University Press 53/1(1989-02), 21-24 0016-6723 53:1<21 1989 53 GRH https://doi.org/10.1017/S0016672300027828 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0016672300027828 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Garside William Department of Biology, Temple University, Philadelphia, PA 19122 NATIONALLICENCE 700 1- Hillman Nina Department of Biology, Temple University, Philadelphia, PA 19122 NATIONALLICENCE 773 0- Genetical Research Cambridge University Press 53/1(1989-02), 21-24 0016-6723 53:1<21 1989 53 GRH CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge