caa a22 4500 386393036 CHVBK 20180307112126.0 cr unu---uuuuu 161130e198908 xx s 000 0 eng 10.1017/S0016672300028305 doi S0016672300028305 pii (NATIONALLICENCE)cambridge-10.1017/S0016672300028305 Isolation of Bacillus subtilis transformation-deficient mutants and mapping of competence genes [Elektronische Daten] We have isolated and characterized 48 Bacillus subtilis competence-deficient mutants. The mutants, obtained by nitrososoguanidine mutagenesis or by insertional mutagenesis with transposon Tn917, had a reduced transformation frequency and a wild-type transduction frequency. The com mutations were mapped by PBS1 transduction and at least four new com genes have been identified. The mutants were also characterized for their capacity to bind and take up the transforming DNA. Copyright © Cambridge University Press 1989 Mastromei Giorgio Universitä degli studi di Firenze, Department of Animal Biology and Genetics, via Romana 17, 50125 Firenze, Italy Barberio Claudia Universitä degli studi di Firenze, Department of Animal Biology and Genetics, via Romana 17, 50125 Firenze, Italy Pistolesi Stefania Universitä degli studi di Firenze, Department of Animal Biology and Genetics, via Romana 17, 50125 Firenze, Italy Polsinelli Mario Universitä degli studi di Firenze, Department of Animal Biology and Genetics, via Romana 17, 50125 Firenze, Italy Genetical Research Cambridge University Press 54/1(1989-08), 1-6 0016-6723 54:1<1 1989 54 GRH https://doi.org/10.1017/S0016672300028305 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0016672300028305 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mastromei Giorgio Universitä degli studi di Firenze, Department of Animal Biology and Genetics, via Romana 17, 50125 Firenze, Italy NATIONALLICENCE 700 1- Barberio Claudia Universitä degli studi di Firenze, Department of Animal Biology and Genetics, via Romana 17, 50125 Firenze, Italy NATIONALLICENCE 700 1- Pistolesi Stefania Universitä degli studi di Firenze, Department of Animal Biology and Genetics, via Romana 17, 50125 Firenze, Italy NATIONALLICENCE 700 1- Polsinelli Mario Universitä degli studi di Firenze, Department of Animal Biology and Genetics, via Romana 17, 50125 Firenze, Italy NATIONALLICENCE 773 0- Genetical Research Cambridge University Press 54/1(1989-08), 1-6 0016-6723 54:1<1 1989 54 GRH CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge