caa a22 4500 386393389 CHVBK 20180307112127.0 cr unu---uuuuu 161130e198910 xx s 000 0 eng 10.1017/S0016672300028500 doi S0016672300028500 pii (NATIONALLICENCE)cambridge-10.1017/S0016672300028500 P-element transposase induces male recombination in Drosophila melanogaster [Elektronische Daten] Male recombination in P-M dysgenic crosses has been viewed as a reflection of P-element transposase interacting with P elements. However, recent studies suggest that the transposase may catalyse double-stranded breaks in chromosomal DNA. We have, therefore, introduced P(Δ2-3 ry+) (99B), a single non-mobile P-element transposase source, into the long-standing laboratory true M strains of a flanking lethal crossover selective system, thus facilitating the examination of rare male recombination events as an assay for transposase activity. We find that the rate of male recombination in the presence of this non-mobile P element is greater than twenty times the background rate of male recombination in the control examined prior to introduction of the transposase source. Copyright © Cambridge University Press 1989 McCarron Margaret Y. Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA Duttaroy Atanu Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA Doughty Glenn A. Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA Chovnick Arthur Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA Genetical Research Cambridge University Press 54/2(1989-10), 137-142 0016-6723 54:2<137 1989 54 GRH https://doi.org/10.1017/S0016672300028500 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0016672300028500 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McCarron Margaret Y. Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA NATIONALLICENCE 700 1- Duttaroy Atanu Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA NATIONALLICENCE 700 1- Doughty Glenn A. Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA NATIONALLICENCE 700 1- Chovnick Arthur Department of Molecular and Cell Biology, The University of Connecticut, Storrs, CT 06269-2131, USA NATIONALLICENCE 773 0- Genetical Research Cambridge University Press 54/2(1989-10), 137-142 0016-6723 54:2<137 1989 54 GRH CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge