caa a22 4500 386393583 CHVBK 20180307112128.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S0016672300028172 doi S0016672300028172 pii (NATIONALLICENCE)cambridge-10.1017/S0016672300028172 Cytogenetic manifestations associated with the reversion, by gene amplification, at the HGPRT locus in V79 Chinese hamster cells [Elektronische Daten] Some HGPRT spontaneous revertants were isolated from a mutant line (E2) of V79 Chinese hamster cells and phenotypically characterized. Dot-Blot hybridization with a 32P-Iabelled HGPRT probe revealed an increase in the number of HGPRT sequences in some of these revertants, suggesting the occurrence of gene amplification. Cytogenetic analysis performed in three of these revertants showed a characteristic abnormally banding region (ABR) on the elongated p arm of the X chromosome. In Situ hybridization in one revertant (RHE2) showed that the amplified sequences reside on the p+ arm of the X chromsome in two different localizations. Because of the very probable clonal origin of the revertant, these features indicate that the amplified sequences might rearrange after their integration into the chromosome. Copyright © Cambridge University Press 1989 Di Leonardo A. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy Agnese C. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy Cavolina P. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy Maddalena A. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy Sciandrello G. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy Randazzo R. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy Genetical Research Cambridge University Press 53/3(1989-06), 201-206 0016-6723 53:3<201 1989 53 GRH https://doi.org/10.1017/S0016672300028172 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0016672300028172 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Di Leonardo A. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy NATIONALLICENCE 700 1- Agnese C. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy NATIONALLICENCE 700 1- Cavolina P. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy NATIONALLICENCE 700 1- Maddalena A. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy NATIONALLICENCE 700 1- Sciandrello G. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy NATIONALLICENCE 700 1- Randazzo R. Department of Cell and Developmental Biology ‘A. Monroy', University of Palermo, via Archiraft 22, 90123 Palermo, Italy NATIONALLICENCE 773 0- Genetical Research Cambridge University Press 53/3(1989-06), 201-206 0016-6723 53:3<201 1989 53 GRH CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge