caa a22 4500 386398976 CHVBK 20180307112151.0 cr unu---uuuuu 161130e198908 xx s 000 0 eng 10.1017/S0031182000072139 doi S0031182000072139 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000072139 Antigen shedding from the surface of the infective stage larvae of Dirofilaria immitis [Elektronische Daten] A qualitative and quantitative analysis was made of the release of surface-associated molecules from developing Dirofilaria immitis infective-stage larvae (L3). D. immitis L3s were labelled with 125I using an Iodogen catalysed reaction and either maintained in culture or placed in chambers that were implanted into Lewis rats. The larvae released 10-20% of the labelled material each day during the first 4 days of in vitro and in vivo development. The loss of surface-labelled peptides from developing larvae corresponded with an increase in the amount of trichloroacetic acid-precipitable radioactivity found in the culture medium. SDS—PAGE analysis of the labelled material showed that the same 35 and 6 kDa components found in larval extracts were shed into culture medium by the developing parasites. Metabolic labelling studies and experiments in which larvae were labelled after different times in culture indicated that, once released, the surfaceassociated molecules were not replaced, and that this net loss of surface peptides resulted in a reduction in the antigenic potential of the cuticular surface. Antibodies from both immunized rabbits and naturally infected dogs immunoprecipitated the 35 kDa component. In contrast, the 6 kDa molecule was not recognized by the antibodies in any of the sera tested. Shedding of surface peptides and reducing surface antigenicity may represent mechanisms by which D. immitis infective-stage larvae evade immune attack. Copyright © Cambridge University Press 1989 Dirofilaria immitis nationallicence infective-stage larvae nationallicence surface-associated antigens nationallicence antigen shedding nationallicence evasion of the immune response nationallicence Ibrahim M. S. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205 Tamashiro W. K. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205 Moraga D. A. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205 Scott A. L. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205 Parasitology Cambridge University Press 99/S1(1989-08), 89-97 0031-1820 99:S1<89 1989 99 PAR https://doi.org/10.1017/S0031182000072139 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000072139 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ibrahim M. S. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205 NATIONALLICENCE 700 1- Tamashiro W. K. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205 NATIONALLICENCE 700 1- Moraga D. A. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205 NATIONALLICENCE 700 1- Scott A. L. Department of Immunology and Infectious Diseases, The Johns Hopkins University School of Hygiene and Public Health, 615 North Wolfe Street, Baltimore, MD 21205 NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/S1(1989-08), 89-97 0031-1820 99:S1<89 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 386398976 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge