caa a22 4500 386399069 CHVBK 20180307112151.0 cr unu---uuuuu 161130e198908 xx s 000 0 eng 10.1017/S0031182000072061 doi S0031182000072061 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000072061 Integration and expression of murine retrovirus-related sequences in schistosomes [Elektronische Daten] Antibodies against the retrovirus envelope glycoprotien (gp70) of mouse xenotropic retrovirus, BALB virus 2 (Bv2) reacted with the adult worms of Schistosoma japonicum and S. mansoni. This reaction was completely inhibited after adsorption of the antibodies with virions of retrovirus. The reactive schistosome antigen was located in the subtegumental layer of the adult male fluke and in the vitelline gland of the adult female of S. japonicum and S. mansoni. Proteins extracted from both parasites were examined by immunoblot analysis. Anti-Bv2 gp70 antiserum reacted with those proteins from both schistosomes and the band patterns were different among sexes and species. Southern hybridization of the DNA extracted from adults of S. japonicum and S. mansoni demonstrated the presence of sequences homologous to the env gene of mouse ecotropic and xenotropic retroviruses. DNA sequences homologous to the gag and pol regions of the ecotropic murine leukaemia virus were also detected in the DNAs of schistosomes Copyright © Cambridge University Press 1989 Schistosoma japonicum nationallicence Schistosoma mansoni nationallicence BALB virus 2 nationallicence retrovirus nationallicence envelope glycoproteins (gp70) nationallicence antigens nationallicence Tanaka M. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan Iwamura Y. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan Amanuma H. The Institute of Physical and Chemical Research, Tsukuba, Ibaraki 305, Japan Irie Y. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan Watanabe M. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan Watanabe T. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan Uchiyama Y. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan Yasuraoka K. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan Parasitology Cambridge University Press 99/S1(1989-08), 31-38 0031-1820 99:S1<31 1989 99 PAR https://doi.org/10.1017/S0031182000072061 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000072061 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanaka M. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan NATIONALLICENCE 700 1- Iwamura Y. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan NATIONALLICENCE 700 1- Amanuma H. The Institute of Physical and Chemical Research, Tsukuba, Ibaraki 305, Japan NATIONALLICENCE 700 1- Irie Y. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan NATIONALLICENCE 700 1- Watanabe M. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan NATIONALLICENCE 700 1- Watanabe T. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan NATIONALLICENCE 700 1- Uchiyama Y. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan NATIONALLICENCE 700 1- Yasuraoka K. The Institute of Basic Medical Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/S1(1989-08), 31-38 0031-1820 99:S1<31 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 386399069 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge