caa a22 4500 386399085 CHVBK 20180307112151.0 cr unu---uuuuu 161130e198908 xx s 000 0 eng 10.1017/S0031182000072140 doi S0031182000072140 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000072140 Neurosecretory-like material in 3rd- and 4th-stage Dirofilaria immitis larvae (Nematoda: Filarioidea) [Elektronische Daten] Phase-interference microscopic examination of the infective, post-infective 3rd-stage and 4th-stage larvae of Dirofilaria immitis has identified a single cell body in each of the paired lateral amphidial nerves which undergoes characteristic morphological change during the development from 3rd- to 4th-stage larvae. Acetaldehyde—fuchsin staining of worm sections revealed fuchsinophilic material in the precise location of the amphidial nerve-cell bodies observed by phaseinterference microscopy. This material was found in 70% of infective larvae recovered from mosquitoes and in 100% of larvae recovered from micropore chambers 24 h after implantation into BALB/C mice. In pre-moult larvae recovered at 42 h (Experiment 1) and at 48 h (Experiment 2) fuchsinophilic material was demonstrable, but no staining was observed in those larvae in which separation of the 3rd- and 4th-stage cuticles had occurred. No such material was observed in 4thstage larvae recovered from chambers after 74 h, and in these larvae the amphidial nerve-cell bodies were not discernible. These cytological observations are consistent with a cycle of elaboration and release of neurosecretion associated with moulting in D. immitis. Copyright © Cambridge University Press 1989 Dirofilaria immitis nationallicence neurosecretion nationallicence moulting nationallicence infective larvae nationallicence Delves C. J. Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA Webb R. Department of Zoology, University of Sheffield, Sheffield S10 2TN Howells R. E. Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA Parasitology Cambridge University Press 99/S1(1989-08), 99-104 0031-1820 99:S1<99 1989 99 PAR https://doi.org/10.1017/S0031182000072140 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000072140 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Delves C. J. Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA NATIONALLICENCE 700 1- Webb R. Department of Zoology, University of Sheffield, Sheffield S10 2TN NATIONALLICENCE 700 1- Howells R. E. Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/S1(1989-08), 99-104 0031-1820 99:S1<99 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 386399085 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge