caa a22 4500 386399093 CHVBK 20180307112151.0 cr unu---uuuuu 161130e198908 xx s 000 0 eng 10.1017/S0031182000072073 doi S0031182000072073 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000072073 Fate of attenuated schistosomula administered to mice by different routes, relative to the immunity induced against Schistosoma mansoni [Elektronische Daten] Newly transformed schistosomula and day 8 lung parasites, derived from optimally irradiated cercariae, were used to immunize groups of C57B1/6 mice via 4 different injection routes. Schistosomula administered intradermally induced high levels of protection, comparable with that achieved by percutaneous vaccination. Intermediate levels were elicited by delivery of parasites via intraperitoneal or intratracheal routes. In contrast, intravenous injection of schistosomula to the lungs resulted in little or no resistance. Attenuated day 8 schistosomula administered intradermally were at least as immunogenic as irradiated cercariae. The fate of radio-isotope labelled attenuated lung schistosomula, injected via the various routes, was examined by compressed organ autoradiography. After intradermal vaccination, a proportion of parasites migrated from the site of injection to the draining lymph node and lungs. Conversely, schistosomula administered via the other 3 routes persisted to varying degrees at the injection site, but little onward migration was observed. We suggest that successful vaccination requires that some attenuated parasites migrate to, and sequester in, lymph nodes draining the vaccination site; persistence at the site of administration alone is not an adequate stimulus. Copyright © Cambridge University Press 1989 Schistosoma mansoni nationallicence attenuated parasite nationallicence injection route nationallicence protective immunity nationallicence Coulson Patricia S. Department of Biology, University of York, York Y01 5DD Mountford A. P. Department of Biology, University of York, York Y01 5DD Parasitology Cambridge University Press 99/S1(1989-08), 39-45 0031-1820 99:S1<39 1989 99 PAR https://doi.org/10.1017/S0031182000072073 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000072073 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Coulson Patricia S. Department of Biology, University of York, York Y01 5DD NATIONALLICENCE 700 1- Mountford A. P. Department of Biology, University of York, York Y01 5DD NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/S1(1989-08), 39-45 0031-1820 99:S1<39 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 386399093 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge