caa a22 4500 386399182 CHVBK 20180307112151.0 cr unu---uuuuu 161130e198908 xx s 000 0 eng 10.1017/S0031182000061126 doi S0031182000061126 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061126 Theilerial parasites isolated from carrier cattle after immunization with Theileria parva by the infection and treatment method [Elektronische Daten] Groups of cattle were immunized with 10−2 dilutions of sporozoite stabilates of Theileria parva lawrencei derived from African buffaloes either alone or in combination with Theileria parva parva derived from cattle and concomitant treatment with either long or short-acting formulations of oxytetracyline. At 90 or 120 days after infection, uninfected Rhipicephalus appendiculatus nymphal ticks were applied to individual immunized cattle and the resultant adults ticks were applied to individual susceptible cattle. Theilerial infection developed from ticks fed on 6 out of 11 animals investigated for evidence of a carrier state. Two additional animals were shown by cell-culture isolation to have persistent theilerial infections. Nine cattle infected with the parasites from carrier animals were treated with parvaquone and 7 recovered. These recovered cattle were then challenged with the original immunizing stabilates at 10° dilution together with the original immunized and carrier cattle. Six out of 7 cattle which had recovered from carrier-derived infection succumbed to this challenge and died but none of the original immunized cattle showed theilerial reactions. When a carrier-derived sporozoite stabilate was used to challenge cattle immune to the original immunizing parasite, they proved to be immune. Cattle immune to the carrier-derived parasites were all immune to challenge with the original parasite. A monoclonal antibody profile aginst T. parva schizonts isolated by cell culture from samples of the experimental animals did not appear to be sensitive enough to determine the antigenic differences between the carrier-derived parasite and the original immunizing parasite. Indications are that the carrier state is not likely to produce new antigenic strains which would be dangerous to immunized cattle. Copyright © Cambridge University Press 1989 Theileria parva nationallicence Rhipicephalus appendiculatus nationallicence carrier parasite nationallicence antigenic differences nationallicence Maritim A. C. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Young A. S. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Lesan A. C. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Ndungu S. G. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Mutugi J. J. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Stagg D. A. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Parasitology Cambridge University Press 99/1(1989-08), 139-147 0031-1820 99:1<139 1989 99 PAR https://doi.org/10.1017/S0031182000061126 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061126 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maritim A. C. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 700 1- Young A. S. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 700 1- Lesan A. C. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 700 1- Ndungu S. G. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 700 1- Mutugi J. J. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 700 1- Stagg D. A. Protozoology Division, National Veterinary Research Centre, Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/1(1989-08), 139-147 0031-1820 99:1<139 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 386399182 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge