caa a22 4500 386399204 CHVBK 20180307112151.0 cr unu---uuuuu 161130e198908 xx s 000 0 eng 10.1017/S0031182000061023 doi S0031182000061023 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061023 Detection of Trypanosoma congolense and Trypanosoma brucei subspecies by DNA amplification using the polymerase chain reaction [Elektronische Daten] The nuclear DNA of Trypanosoma congolense contains a family of highly conserved 369 base pair (bp) repeats. The sequences of three cloned copies of these repeats were determined. An unrelated family of 177 bp repeats has previously been shown to occur in the nuclear DNA of Trypanosoma brucei brucei (Sloof et al. 1983a). Oligonucleotides were synthesized which prime the specific amplification of each of these repetitive DNAs by the polymerase chain reaction (PCR). Amplification of 10% of the DNA in a single parasite of T. congolense or T. brucei spp. produced sufficient amplified product to be visible as a band in an agarose gel stained with ethidium bromide. This level of detection, which does not depend on the use of radioactivity, is about 100 times more sensitive than previous detection methods based on radioactive DNA probes. The oligonucleotides did not prime the amplification of DNA sequences in other trypanosome species nor in Leishmania, mouse or human DNAs. Amplification of DNA from the blood of animals infected with T. congolense and/or T. brucei spp. permitted the identification of parasite levels far below that detectable by microscopic inspection. Since PCR amplification can be conducted on a large number of samples simultaneously, it is ideally suited for large-scale studies on the prevalence of African trypanosomes in both mammalian blood and insect vectors. Copyright © Cambridge University Press 1989 Trypanosoma congolense nationallicence Trypanosoma brucei nationallicence DNA amplification nationallicence polymerase chain reaction nationallicence Moser D. R. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 Cook G. A. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 Ochs Diane E. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 Bailey Cheryl P. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 McKane Melissa R. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 Donelson J. E. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 Parasitology Cambridge University Press 99/1(1989-08), 57-66 0031-1820 99:1<57 1989 99 PAR https://doi.org/10.1017/S0031182000061023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Moser D. R. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 NATIONALLICENCE 700 1- Cook G. A. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 NATIONALLICENCE 700 1- Ochs Diane E. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 NATIONALLICENCE 700 1- Bailey Cheryl P. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 NATIONALLICENCE 700 1- McKane Melissa R. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 NATIONALLICENCE 700 1- Donelson J. E. Department of Biochemistry, and Diabetes and Endocrinology Research Center, University of Iowa, Iowa City, IA 52242 NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/1(1989-08), 57-66 0031-1820 99:1<57 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence SWISSBIB 386399107 BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge