caa a22 4500 386399336 CHVBK 20180307112152.0 cr unu---uuuuu 161130e198902 xx s 000 0 eng 10.1017/S0031182000059746 doi S0031182000059746 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059746 Egg production in Brugia pahangi(Nematoda: Filarioidea) [Elektronische Daten] Oogenesis in Brugia pahangi has been studied by means of the aceto-orcein chromosomal squash technique and light-microscope autoradiography. The use of colchicine has demonstrated a 2-3 mm terminal germinative zone within the ovary, in which continuous and rapid mitotic division of germ cells occurs. In 80% of the gonads, oocytes within a 1-2 mm length of the ovary proximal to the germinative zone were at the prophase of meiosis I. Primary oocytes with markedly less condensed chromatin, apparently interphase cells, were observed in the corresponding region of the ovary in the remaining 20% of material examined. A cyclical or phased development of primary oocytes is suggested. Autoradiographic studies, concerned with the incorporation of [5-3H]uridine into germ cells of B. pahangi in vitro, further suggest that the onset of meiotic prophase is associated with the initiation of high RNA synthetic activity. Following meiotic prophase, oocytes complete meiosis I before entering a period of growth during which the chromatin material is decondensed. Recondensation of chromosomes prior to meiosis II is only observed after fertilization within the seminal receptacle. On completion of meiosis II, with the extrusion of a polar body, the haploid chromosome complement of the female unites with that of the male, re-establishing the diploid number of the zygote (2n = 10). Copyright © Cambridge University Press 1989 Delves C. J. Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA Rees H. H. Department of Biochemistry, University of Liverpool Howells R. E. Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA Parasitology Cambridge University Press 98/1(1989-02), 105-113 0031-1820 98:1<105 1989 98 PAR https://doi.org/10.1017/S0031182000059746 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059746 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Delves C. J. Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA NATIONALLICENCE 700 1- Rees H. H. Department of Biochemistry, University of Liverpool NATIONALLICENCE 700 1- Howells R. E. Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/1(1989-02), 105-113 0031-1820 98:1<105 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge