caa a22 4500 386399344 CHVBK 20180307112152.0 cr unu---uuuuu 161130e198902 xx s 000 0 eng 10.1017/S0031182000059801 doi S0031182000059801 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059801 Factors affecting the adhesion of micro-organisms to the surfaces of plant-parasitic nematodes [Elektronische Daten] The influence of various agents on the adhesion of endospores of Pasteuria penetrans to the nematode Meloidogyne javanica was studied. Similarly, but to a lesser degree, we have also studied the adhesion of conidia of the fungus Dilophospora alopecuri and the coryneform bacterium Clavibacter sp. (syn. Corynebacterium rathayi) to the nematode Anguina agrostis (syn. A. funesta). Reduction in the degree of both spore and conidial attachment following their pre-treatment with periodate and the presence of PAS staining material on spores, conidia and bacteria implicated carbohydrate in these interactions. Tests involving both unbound and FITC-bound lectins demonstrated that wheat germ agglutinin (WGA) can inhibit the degree of attachment of P. penetrans to M. javanica and that this inhibition can be overcome by pre-treatment of the lectin with N, N′-diacetyl chitobiose. Endospores of P. penetrans, amphid and buccal secretions of 2nd-stage larvae of M. javanica and the cuticle and excretory pore secretions of 2nd-stage dauer larvae of A. agrostis bound WGA, indicating that accessible N-acetyl-D-glucosamine residues are present on these structures. Endospores of P. penetrans also bound Con A, indicating the presence of accessible α-D-glucose/α-D-mannose residues on their surface. Copyright © Cambridge University Press 1989 plant nematodes nationallicence adhesion nationallicence micro-organisms nationallicence Bird A. F. CSIRO, Division of Soils, Private Bag No. 2, P.O. Glen Osmond, AU 5064 Bonig Ingrid Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052 Bacic A. Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052 Parasitology Cambridge University Press 98/1(1989-02), 155-164 0031-1820 98:1<155 1989 98 PAR https://doi.org/10.1017/S0031182000059801 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059801 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Bird A. F. CSIRO, Division of Soils, Private Bag No. 2, P.O. Glen Osmond, AU 5064 NATIONALLICENCE 700 1- Bonig Ingrid Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052 NATIONALLICENCE 700 1- Bacic A. Plant Cell Biology Research Centre, School of Botany, University of Melbourne, Parkville, Victoria 3052 NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/1(1989-02), 155-164 0031-1820 98:1<155 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge