caa a22 4500 386399409 CHVBK 20180307112152.0 cr unu---uuuuu 161130e198902 xx s 000 0 eng 10.1017/S0031182000059734 doi S0031182000059734 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059734 Strongyloides ratti: studies of cutaneous reactions elicited in naive and sensitized rats and of changes in surface antigenicity of skin-penetrating larvae [Elektronische Daten] This study provides the first quantitative assessment of the cutaneous reaction elicited in naive and sensitized rats by invading larvae of Strongyloides ratti. Parasites were identified in skin samples harvested from both naive and sensitized rats between 0·5 and 8 h following percutaneous infection, but were no longer visible at the site of invasion at 24 h. Mast cells, neutrophils, eosinophils and mononuclear cells recruited into the dermis in response to invading larvae were recorded and quantified. Cellular infiltrates were observed as early as 1 h post-invasion in naive/challenged skin, where total cell numbers were up to 3 times greater than in naive control tissue. Peak numbers of all cell types were recorded from 3 to 8 h in both naive/challenged and sensitized/challenged hosts. Interestingly, the reaction in sensitized/challenged rats was neither enhanced nor accelerated, a feature perhaps attributable to lack of recognition of parasite antigens by the host. This possibility was investigated by immunofluorescent labelling, which clearly demonstrated changes in the surface antigen profile of the parasite following penetration of the host skin both in vitro and in vivo. It is proposed that these changes in surface antigenicity constitute an evasive stratagem used by the parasite to deter the host from mounting a potentially lethal inflammatory response. Copyright © Cambridge University Press 1989 Strongyloides ratti nationallicence cutaneous reactions nationallicence surface antigenicity nationallicence skin-penetrating larvae nationallicence McHugh T.D. School of Biological Sciences, Portsmouth Polytechnic, Portsmouth PO1 2DY Jenkins T. School of Biological Sciences, Portsmouth Polytechnic, Portsmouth PO1 2DY McLaren Diane J. Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA Parasitology Cambridge University Press 98/1(1989-02), 95-103 0031-1820 98:1<95 1989 98 PAR https://doi.org/10.1017/S0031182000059734 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059734 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- McHugh T.D. School of Biological Sciences, Portsmouth Polytechnic, Portsmouth PO1 2DY NATIONALLICENCE 700 1- Jenkins T. School of Biological Sciences, Portsmouth Polytechnic, Portsmouth PO1 2DY NATIONALLICENCE 700 1- McLaren Diane J. Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/1(1989-02), 95-103 0031-1820 98:1<95 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge