caa a22 4500 386399425 CHVBK 20180307112152.0 cr unu---uuuuu 161130e198902 xx s 000 0 eng 10.1017/S003118200005962X doi S003118200005962X pii (NATIONALLICENCE)cambridge-10.1017/S003118200005962X Relations between resistance to chloroquine and acidification of endocytic vesicle of Plasmodium berghei [Elektronische Daten] In order to visualize low-pH compartments of Plasmodium berghei strains we have used a basic congener of dinitrophenol, 3-(2,4-dinitroanilino)-3'-amino-N-methyldipropylamine (DAMP) which concentrates in acidic compartments, and can be detected by immunocytochemistry with anti-dinitrophenol antibodies. We have demonstrated that in a P. berghei chloroquine-sensitive strain (N strain), DAMP accumulates in the endocytic vacuoles where haemoglobin degradation is occurring. These compartments which have recently been shown to concentrate 4-aminoquinoline drugs (Moreau, Prensier, Maalla & Fortier, 1986) have an acidic pH. Conversely DAMP was found scattered all over the cytoplasm in a P. berghei chloroquine-resistant strain; the same phenomenon was previously observed (Moreau et al. 1986) in the localization of a 4-aminoquinoline on this same strain. Monensin-induced swelling of acidic compartments (Boss & Morre, 1984) was used as a complementary method for the determination of low-pH compartments on P. berghei strains. All the data reported here suggest that chloroquine resistance in P. berghei RC may be related to an impairment in the acidification of endocytic vesicles. Copyright © Cambridge University Press 1989 Plasmodium berghei nationallicence chloroquine resistance nationallicence endocytic vesicle nationallicence acidic compartments nationallicence Mahmalgi J. INSERM U.42, Biologie et Biochimie Parasitaire et Fongique, Villeneuve d'Ascq, FR Veignie E. INSERM U.42, Biologie et Biochimie Parasitaire et Fongique, Villeneuve d'Ascq, FR Prensier G. INSERM U.42, Biologie et Biochimie Parasitaire et Fongique, Villeneuve d'Ascq, FR Moreau S. INSERM U.42, Biologie et Biochimie Parasitaire et Fongique, Villeneuve d'Ascq, FR Parasitology Cambridge University Press 98/1(1989-02), 1-6 0031-1820 98:1<1 1989 98 PAR https://doi.org/10.1017/S003118200005962X text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S003118200005962X text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mahmalgi J. INSERM U.42, Biologie et Biochimie Parasitaire et Fongique, Villeneuve d'Ascq, FR NATIONALLICENCE 700 1- Veignie E. INSERM U.42, Biologie et Biochimie Parasitaire et Fongique, Villeneuve d'Ascq, FR NATIONALLICENCE 700 1- Prensier G. INSERM U.42, Biologie et Biochimie Parasitaire et Fongique, Villeneuve d'Ascq, FR NATIONALLICENCE 700 1- Moreau S. INSERM U.42, Biologie et Biochimie Parasitaire et Fongique, Villeneuve d'Ascq, FR NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/1(1989-02), 1-6 0031-1820 98:1<1 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge