caa a22 4500 386399433 CHVBK 20180307112152.0 cr unu---uuuuu 161130e198902 xx s 000 0 eng 10.1017/S0031182000059710 doi S0031182000059710 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059710 Cloning of a repetitive DNA from the rickettsia-like organisms of tsetse flies (Glossina spp.) [Elektronische Daten] Three DNA fragments from the genome of the rickettsia-like organism (RLO) symbiotic in tsetse flies (Glossina spp.) have been cloned. One of these fragments represents a family of highly conserved repeats, which occurs in RLO from all species of tsetse examined and has been amplified in the original RLO culture from which the DNA is cloned. This fragment serves as a highly sensitive and specific probe for the detection of RLO in tsetse midguts. As few as 30 organisms were unequivocally identified by dot blot hybridization of homogenized midgut preparations. Since the presence of RLO within tsetse midguts is associated with susceptibility to trypanosome infection, this technique provides a rapid and reliable method of assessing the potential susceptibility of a tsetse population to Trypanosoma brucei s.l. and T. congolense infections. Copyright © Cambridge University Press 1989 DNA probe nationallicence rickettsia nationallicence tsetse nationallicence trypanosome nationallicence wolbachia nationallicence Welburn S. C. Tsetse Research Laboratory, ODA/University of Bristol, Langford, Bristol BS18 7DU Gibson W. C. Department of Pathology, University of Bristol, Langford, Bristol BS18 7DU Parasitology Cambridge University Press 98/1(1989-02), 81-84 0031-1820 98:1<81 1989 98 PAR https://doi.org/10.1017/S0031182000059710 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059710 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Welburn S. C. Tsetse Research Laboratory, ODA/University of Bristol, Langford, Bristol BS18 7DU NATIONALLICENCE 700 1- Gibson W. C. Department of Pathology, University of Bristol, Langford, Bristol BS18 7DU NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/1(1989-02), 81-84 0031-1820 98:1<81 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge