caa a22 4500 386399468 CHVBK 20180307112152.0 cr unu---uuuuu 161130e198902 xx s 000 0 eng 10.1017/S0031182000059679 doi S0031182000059679 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059679 Schistosoma mansoni: circulating and pulmonary leucocyte responses related to the induction of protective immunity in mice by irradiated parasites [Elektronische Daten] The leucocyte responses in peripheral blood and pulmonary airways are described following vaccination of mice with radiation-attenuated parasites, and subsequent challenge with normal parasites. Percutaneous vaccination stimulated a large and sustained expansion of the circulating lymphocyte pool, more marked than after intradermal vaccination with lung schistosomula which induced comparable levels of resistance. Macrophages and lymphocytes infiltrated the pulmonary airways in response to vaccination by both routes, the lymphocytes being particularly abundant after intradermal vaccination. Exposure of mice to an equivalent number of normal cercariae induced an earlier lymphocytosis of short duration; far fewer macrophages and lymphocytes infiltrated the lungs than after vaccination. An intense but transient pulmonary eosinophilia peaked at 3 weeks after primary exposure to either normal or attenuated parasites. Percutaneous challenge of vaccinated mice elicited higher levels of circulating lymphocytes than challenge of unsensitized controls. However, whilst leucocyte numbers of all cell types were still elevated in the airways at challenge as a consequence of vaccination, no further cellular recruitment was observed coincident with parasite elimination. Our data are compatible with the hypothesis that the mechanism of immunity in once-vaccinated mice involves a T lymphocyte-macrophage interaction triggered by antigen release from lung schistosomula. Copyright © Cambridge University Press 1989 Schistosoma mansoni nationallicence leucocytes nationallicence protective immunity nationallicence irradiation nationallicence Menson Essie N. Department of Biology, University of York, York YO1 5DD Coulson Patricia S. Department of Biology, University of York, York YO1 5DD Wilson R. A. Department of Biology, University of York, York YO1 5DD Parasitology Cambridge University Press 98/1(1989-02), 43-55 0031-1820 98:1<43 1989 98 PAR https://doi.org/10.1017/S0031182000059679 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059679 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Menson Essie N. Department of Biology, University of York, York YO1 5DD NATIONALLICENCE 700 1- Coulson Patricia S. Department of Biology, University of York, York YO1 5DD NATIONALLICENCE 700 1- Wilson R. A. Department of Biology, University of York, York YO1 5DD NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/1(1989-02), 43-55 0031-1820 98:1<43 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge