caa a22 4500 386399476 CHVBK 20180307112152.0 cr unu---uuuuu 161130e198902 xx s 000 0 eng 10.1017/S0031182000059709 doi S0031182000059709 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059709 Differentiation of Schistosoma mansoni from S. rodhaini using cloned DNA probes [Elektronische Daten] The ribosomal RNA (rRNA) gene units of Schistosoma mansoni and S. rodhaini, of the lateral spined egg group, have been studied. The schistosome rRNA gene unit consists of a regular interspersion of the two genes encoding the large and small rRNA units with two spacers. The large spacer is not transcribed while the small spacer is part of the transcription unit. Variation in the rRNA gene unit between the two species is demonstrated to take the form of loss or gain of restriction sites within the non-transcribed and transcribed spacers. This variation has been demonstrated to enable the differentiation of S. mansoni from S. rodhaini by Southern hybridization analysis. In addition, a DNA clone representing a female specific, tandemly repeated sequence, is demonstrated to enable differentiation of S. mansoni and S. rodhaini using dot blot hybridization analysis. Copyright © Cambridge University Press 1989 Schistosoma spp nationallicence differentiation nationallicence cloned DNA probes nationallicence Walker Tina K. Department of Zoology, British Museum (Natural History), Cromwell Road, South Kensington, London SW7 5BD Simpson A. J. G. Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA Rollinson D. Department of Zoology, British Museum (Natural History), Cromwell Road, South Kensington, London SW7 5BD Parasitology Cambridge University Press 98/1(1989-02), 75-80 0031-1820 98:1<75 1989 98 PAR https://doi.org/10.1017/S0031182000059709 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059709 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Walker Tina K. Department of Zoology, British Museum (Natural History), Cromwell Road, South Kensington, London SW7 5BD NATIONALLICENCE 700 1- Simpson A. J. G. Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 700 1- Rollinson D. Department of Zoology, British Museum (Natural History), Cromwell Road, South Kensington, London SW7 5BD NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/1(1989-02), 75-80 0031-1820 98:1<75 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge