caa a22 4500 386399824 CHVBK 20180307112154.0 cr unu---uuuuu 161130e198910 xx s 000 0 eng 10.1017/S0031182000058650 doi S0031182000058650 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000058650 Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype [Elektronische Daten] Different stocks and stabilates within a stock of Theileriaparva were analysed for genotypic differences and for their effect on the expression of host cell surface antigens following infection of BoT8+ T lymphocyte clones. The parasites were characterized in vitro by hybridization of T. parva- specific DNA probes to Southern blots of endonuclease-digested DNA from the infected T cell clones. Phenotypic changes in the host lymphoblastoid cells before and after infection were examined using lineage-specific monoclonal antibodies which reacted with the differentiation antigens BoT2, BoT4, Bo6, BoT8 and a null cell marker on bovine T cells. Expression of Class I and Class II major histocompatibility complex (MHC) antigens on the cell populations was also assessed. Results of this study indicate that genotypically different parasites exist among and within T. parva stabilates and that the expression of Bo6, BoT8 and the null cell marker was differentially altered by infection with parasites from different stocks or from different stabilates of the same stock. Expression of Class II antigens was significantly increased after infection. Moreover, clones that were derived from the same cell line but had genotypically distinct T. parva parasites, also showed differences in expression of Bo6 and BoT8 and the null cell marker. Copyright © Cambridge University Press 1989 Theileria parva nationallicence T lymphocytes nationallicence differentiation antigen nationallicence major histocompatibility antigens nationallicence DNA probes nationallicence Conrad P. A. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya Baldwin C. L. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya Brown W. C. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya Sohanpal B. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya Dolan T. T. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya Goddeeris B. M. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya Demartini J. C. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya Ole-Moiyoi O. K. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya Parasitology Cambridge University Press 99/2(1989-10), 205-213 0031-1820 99:2<205 1989 99 PAR https://doi.org/10.1017/S0031182000058650 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000058650 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Conrad P. A. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Baldwin C. L. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Brown W. C. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Sohanpal B. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Dolan T. T. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Goddeeris B. M. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Demartini J. C. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Ole-Moiyoi O. K. International Laboratory for Research on Animal Diseases (ILRAD), P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/2(1989-10), 205-213 0031-1820 99:2<205 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge