caa a22 4500 386399840 CHVBK 20180307112154.0 cr unu---uuuuu 161130e198910 xx s 000 0 eng 10.1017/S0031182000058686 doi S0031182000058686 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000058686 Origin, kinetics of circulation and fate in vivo of the major excretory-secretory product of Acanthocheilonema viteae [Elektronische Daten] The excretions-secretions (E-S) of Acanthocheilonema viteae consist mainly of one product, molecular weight 62 kDa. This molecule is synthesized during the vertebrate phase of the parasite life-cycle and is first detectable in the E-S of L4 parasites. It is cross-reactive with E-S of human filarial parasites as a consequence of possessing a phosphorylcholine (PC) moiety. The 62 kDa molecule has been employed as a model for the study of the origin and fate of filarial E-S. Immunohistological analysis has shown the molecule to be located predominantly in the parasite gut. Transplantation of adult female [S] methionine pulsed worms into uninfected jirds resulted in the radio-labelled secreted 62 kDa antigen being detected in the bloodstream within 4 h by SDS-PAGE/immunoprecipitation analysis. The systemic half-life of the molecule as estimated by clearance of injected, purified I-labelled material was measured in naive and infected jird hosts. Copyright © Cambridge University Press 1989 Acanthocheilonema viteae nationallicence excretory-secretory product nationallicence kinetics of circulation nationallicence Harnett W. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA Worms M. J. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA Kapil A. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA Grainger M. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA Parkhouse R. M. E. Divisions of Parasitology and Immunology, The Ridgeway, Mill Hill, London NW7 1AA Parasitology Cambridge University Press 99/2(1989-10), 229-239 0031-1820 99:2<229 1989 99 PAR https://doi.org/10.1017/S0031182000058686 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000058686 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Harnett W. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 700 1- Worms M. J. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 700 1- Kapil A. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 700 1- Grainger M. National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 700 1- Parkhouse R. M. E. Divisions of Parasitology and Immunology, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/2(1989-10), 229-239 0031-1820 99:2<229 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge