caa a22 4500 386399875 CHVBK 20180307112154.0 cr unu---uuuuu 161130e198910 xx s 000 0 eng 10.1017/S0031182000058728 doi S0031182000058728 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000058728 Developmental changes in proteins and glycoproteins revealed by direct radio-iodination of viable Taenia saginata larvae [Elektronische Daten] Direct surface I radio-isotope labelling techniques and SDS—PAGE analysis were used to compare the proteins and lentil—lectin adherent glycoproteins of the bovine stage of viable Taenia saginata larvae at three points in their development, the invasive oncospheres, immature (4-week-old) and mature (12 to 16-week-old) cysticerci. Some proteins and glycoproteins were present on all three of the ages of the parasite examined but there were also distinct age-specific proteins and glycoproteins detected on oncospheres and 4-week-old cysticerci and a marked difference between the protein/glycoprotein profiles of the parasite was apparent at these earlier stages of development and the mature cysticerci. The latter were characterized by the presence of high, 160-200 kDa molecular weight, lysine rich, glycoproteins, whereas small 16 and 18 kDa glycoproteins and a reduction-sensitive 23 kDa glycoprotein were first detected on 4-week-old immature cysticerci. Antigenic characterization of the isotope-labelled proteins and glycoproteins by immunoprecipitation against a panel of clinically defined bovine sera combined with SDS-PAGE analysis indicated that relatively few proteins were precipitated by sera from T. saginata-infected cattle as compared to the glycoproteins. However, both protein and glycoprotein antigens of possible protective and/or diagnostic significance were identified from oncospheres and cysticerci. Copyright © Cambridge University Press 1989 Taenia saginata nationallicence development nationallicence proteins nationallicence glycoproteins nationallicence antigens nationallicence Joshua G. W. P. Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG Harrison L. J. S. Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG Sewell M. M. H. Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG Parasitology Cambridge University Press 99/2(1989-10), 265-274 0031-1820 99:2<265 1989 99 PAR https://doi.org/10.1017/S0031182000058728 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000058728 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Joshua G. W. P. Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG NATIONALLICENCE 700 1- Harrison L. J. S. Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG NATIONALLICENCE 700 1- Sewell M. M. H. Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/2(1989-10), 265-274 0031-1820 99:2<265 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge