caa a22 4500 386399891 CHVBK 20180307112154.0 cr unu---uuuuu 161130e198910 xx s 000 0 eng 10.1017/S0031182000058613 doi S0031182000058613 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000058613 Characterization of coccidial proteins by two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis [Elektronische Daten] Two dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D SDS-PAGE) has been used to produce ‘fingerprint' maps of the proteins from each of the 7 species of Eimeria which infect the chicken. All 7 species could be identified from their array of polypeptides but few differences were detected between strains of the same species. Alterations to the polypeptide array associated with the stage of sporulation of the oocysts were observed. lodination of sporozoites, 2D SDS-PAGE, autoradiography and immunoblotting techniques were combined to identify polypeptides with a surface moiety and those which were antigenic. Copyright © Cambridge University Press 1989 coccidial proteins nationallicence Eimeria spp nationallicence fingerprint maps nationallicence 2D SDS-PAGE nationallicence Sutton C. A. AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA Shirley M. W. AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA Wisher M. H. AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA Parasitology Cambridge University Press 99/2(1989-10), 175-187 0031-1820 99:2<175 1989 99 PAR https://doi.org/10.1017/S0031182000058613 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000058613 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sutton C. A. AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA NATIONALLICENCE 700 1- Shirley M. W. AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA NATIONALLICENCE 700 1- Wisher M. H. AFRC, Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/2(1989-10), 175-187 0031-1820 99:2<175 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge