caa a22 4500 386399980 CHVBK 20180307112155.0 cr unu---uuuuu 161130e198910 xx s 000 0 eng 10.1017/S0031182000058649 doi S0031182000058649 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000058649 Use of two-colour flow cytometry to assess killing of Giardia muris trophozoites by antibody and complement [Elektronische Daten] A two-colour flow cytometry technique was used to assess killing of Giardia muris trophozoites by rabbit anti-trophozoite antibodies and complement. Binding of rabbit antibody to trophozoites and killing of trophozoites were documented by flow cytometry, after incubating the organisms with fluorescein-conjugated anti-rabbit IgG and propidium iodide (PI). Percentages of PI+ (dead) trophozoites ranged from >80%, after incubation with rabbit antiserum and complement, to <30%, after incubation with complement alone. The assay technique may be applicable to studies aimed at determining whether intestinal antibodies from Giardia-infected mammals can kill Giardia trophozoites. Copyright © Cambridge University Press 1989 Giardia muris nationallicence flow cytometry nationallicence trophozoites nationallicence killing nationallicence cytotoxic antibody nationallicence complement nationallicence Heyworth M. F. Cell Biology Section (151E), Veterans Administration Medical Center, 4150 Clement Street, San Francisco, California 94121 and Department of Medicine, University of California, San Francisco, USA Pappo J. Cell Biology Section (151E), Veterans Administration Medical Center, 4150 Clement Street, San Francisco, California 94121 and Department of Medicine, University of California, San Francisco, USA Parasitology Cambridge University Press 99/2(1989-10), 199-203 0031-1820 99:2<199 1989 99 PAR https://doi.org/10.1017/S0031182000058649 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000058649 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Heyworth M. F. Cell Biology Section (151E), Veterans Administration Medical Center, 4150 Clement Street, San Francisco, California 94121 and Department of Medicine, University of California, San Francisco, USA NATIONALLICENCE 700 1- Pappo J. Cell Biology Section (151E), Veterans Administration Medical Center, 4150 Clement Street, San Francisco, California 94121 and Department of Medicine, University of California, San Francisco, USA NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/2(1989-10), 199-203 0031-1820 99:2<199 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge