caa a22 4500 386400024 CHVBK 20180307112155.0 cr unu---uuuuu 161130e198904 xx s 000 0 eng 10.1017/S0031182000062077 doi S0031182000062077 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000062077 Host diet in experimental rodent malaria: a variable which can compromise experimental design and interpretation [Elektronische Daten] Over the past few years several experienced groups studying malaria have encountered significant problems with their particular rodent malaria-host system. This has involved, in some cases, periods during which the recovery of cryo-preserved parasite stocks and growth of bloodstream parasites was markedly inhibited and, in other cases, periods of drastically increased mortality rates. The common factor linking these incidents was that they coincided with alterations in the experimental animal diet used. in the experimental animal diet used. The inhibition of growth of cryopreserved stabilates or bloodstream parasites was abolished by supplementation with p-aminobenzoic acid (PABA) or by changing the diet used. Although the suppressive effects of diets lacking PABA on parasite growth have been known for over 30 years, the variation of PABA levels in modern laboratory animal feed concentrates is not well recognized. We have not established the exact cause of increased mortality, but it has been overcome by changing the diet used. We are documenting our experiences with this potential variable to forewarn workers in other laboratories of possible problems inherent in the use of different diets. Copyright © Cambridge University Press 1989 host diet nationallicence rodent malaria nationallicence p-aminobenzoic acid nationallicence mortality nationallicence Gilks C. F. Tropical Medicine Unit, Nuffield Department of Medicine, John Radcliffe Hospital, Oxford OX3 9DU Jarra W. Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA Harvey-Wood K. Wellcome Parasitology Laboratory, University of Glasgow, Glasgow G61 1QH McLean S. A. Wellcome Parasitology Laboratory, University of Glasgow, Glasgow G61 1QH Schetters T. Department of Cytology and Histology, University of Nijmegen, Nijmegen, Holland Parasitology Cambridge University Press 98/2(1989-04), 175-177 0031-1820 98:2<175 1989 98 PAR https://doi.org/10.1017/S0031182000062077 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000062077 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gilks C. F. Tropical Medicine Unit, Nuffield Department of Medicine, John Radcliffe Hospital, Oxford OX3 9DU NATIONALLICENCE 700 1- Jarra W. Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA NATIONALLICENCE 700 1- Harvey-Wood K. Wellcome Parasitology Laboratory, University of Glasgow, Glasgow G61 1QH NATIONALLICENCE 700 1- McLean S. A. Wellcome Parasitology Laboratory, University of Glasgow, Glasgow G61 1QH NATIONALLICENCE 700 1- Schetters T. Department of Cytology and Histology, University of Nijmegen, Nijmegen, Holland NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/2(1989-04), 175-177 0031-1820 98:2<175 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge