caa a22 4500 386400059 CHVBK 20180307112155.0 cr unu---uuuuu 161130e198904 xx s 000 0 eng 10.1017/S0031182000062132 doi S0031182000062132 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000062132 Immunocytochemical demonstration of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica (Trematoda, Digenea) [Elektronische Daten] The localization and distribution of neuropeptides in the nervous system of the liver fluke, Fasciola hepatica at different stages in the development of the adult fluke have been determined by an indirect immunofluorescence technique, using antisera to 19 vertebrate peptides and the invertebrate neuropeptide, FMRFamide. Positive immunoreactivity was obtained with antisera to pancreatic polypeptide (PP), peptide tyrosine tyrosine (PYY), substance P (SP) and FMRFamide. Cell bodies and nerve fibres immunoreactive to the 4 peptides are present in the anterior ganglia and the 3 pairs of longitudinal nerve cords and their commissures in the central nervous system. In the peripheral nervous system, immunoreactivity occurs in the nerve plexuses supplying the subtegumental musculature, the oral and ventral suckers, and the muscular lining of the male and female reproductive ducts, including the ootype, uterus, cirrus pouch and gonopore. Cells displaying immunoreactivity to PYY and FMRFamide lie amongst the Mehlis' gland cells that surround the ootype. Processes from these cells extend into the wall of the ootype. One group of PP-immunoreactive cells occurs at the junction of the vitelline and ovovitelline ducts, whilst another group is situated at the entrance to the uterus from the ootype. The results are discussed in relation to the possible roles of the peptides in the neurophysiology and egg production of the fluke. Copyright © Cambridge University Press 1989 Fasciola hepatica nationallicence trematode neuropeptides nationallicence immunocytochemistry nationallicence pancreatic polypeptide nationallicence peptide tyrosine tyrosine nationallicence substance P FMRFamide nationallicence Magee R. M. Department of Biology Fairweather I. Department of Biology Johnston C. F. Department of Medicine, The Queen's University, Belfast BT7 1NN Halton D. W. Department of Biology Shaw C. Department of Medicine, The Queen's University, Belfast BT7 1NN Parasitology Cambridge University Press 98/2(1989-04), 227-238 0031-1820 98:2<227 1989 98 PAR https://doi.org/10.1017/S0031182000062132 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000062132 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Magee R. M. Department of Biology NATIONALLICENCE 700 1- Fairweather I. Department of Biology NATIONALLICENCE 700 1- Johnston C. F. Department of Medicine, The Queen's University, Belfast BT7 1NN NATIONALLICENCE 700 1- Halton D. W. Department of Biology NATIONALLICENCE 700 1- Shaw C. Department of Medicine, The Queen's University, Belfast BT7 1NN NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/2(1989-04), 227-238 0031-1820 98:2<227 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge