caa a22 4500 386400091 CHVBK 20180307112155.0 cr unu---uuuuu 161130e198904 xx s 000 0 eng 10.1017/S0031182000062089 doi S0031182000062089 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000062089 Characterization of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes [Elektronische Daten] The characteristics of intra-lymphocytic Theileria isolated from African buffalo and from cattle that were infected with buffalo-derived parasites were evaluated using anti-schizont monoclonal antibodies (mAbs) and DNA probes. Antigenic differences were revealed by the reactivities of 27 mAbs with the buffalo-derived parasites isolated from different animals. Antigenic diversity was also seen with Theileria-infected lymphoblastoid cell isolates taken from the lymph nodes and blood of the same animals. Two DNA probes, selected from a genomic library of T. parva piroplasm DNA cloned in λgt11, showed specific hybridization to parasite DNA in Southern blots of restriction enzyme-digested, lymphoblastoid cells infected with buffalo-derived theilerial parasites. Genotypic differences between the buffalo-derived parasites were revealed by the restriction fragment length polymorphisms seen with hybridization of those probes to DNA from cloned and uncloned Theileria-infected cell lines. The evaluation of theilerial parasites derived from buffalo and from cattle which underwent typical T. p. lawrencei reactions, after being infected with buffalo-derived theilerial parasites, did not show any specific phenotypic or genotypic characteristics of these parasites that would distinguish them from T. p. parva and T. p. bovis parasites. The validity of these subspecies distinctions is discussed. Copyright © Cambridge University Press 1989 Theileria parva nationallicence DNA probes nationallicence buffalo characterization nationallicence monoclonal antibodies nationallicence Conrad P. A. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya Ole-Moiyoi O. K. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya Baldwin C. L. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya Dolan T. T. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya O'Callaghan C. J. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya Njamunggehr R. E. G. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya Grootenhuis J. G. Wildlife Diseases Research Project, Veterinary Research Laboratory, P.O. Kabete, Kenya Stagg D. A. National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Leitch B. L. National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Young A. S. National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya Parasitology Cambridge University Press 98/2(1989-04), 179-188 0031-1820 98:2<179 1989 98 PAR https://doi.org/10.1017/S0031182000062089 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000062089 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Conrad P. A. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Ole-Moiyoi O. K. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Baldwin C. L. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Dolan T. T. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- O'Callaghan C. J. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Njamunggehr R. E. G. International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya NATIONALLICENCE 700 1- Grootenhuis J. G. Wildlife Diseases Research Project, Veterinary Research Laboratory, P.O. Kabete, Kenya NATIONALLICENCE 700 1- Stagg D. A. National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 700 1- Leitch B. L. National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 700 1- Young A. S. National Veterinary Research Centre Muguga, Kenya Agricultural Research Institute, P.O. Box 32, Kikuyu, Kenya NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/2(1989-04), 179-188 0031-1820 98:2<179 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge