caa a22 4500 38640013X CHVBK 20180307112155.0 cr unu---uuuuu 161130e198904 xx s 000 0 eng 10.1017/S0031182000062120 doi S0031182000062120 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000062120 Reactivity of anti-tegument monoclonal antibodies with target epitopes in different worm tissues and developmental stages of Schistosoma mansoni [Elektronische Daten] Sixteen monoclonal antibodies (MABs) were selected for their reactivity with adult schistosome tegument. The distribution of target epitopes in different tissues of the adult and in various developmental stages was investigated by indirect immunofluorescence. The distinct patterns of reactivity of the MABs permitted their classification into 9 groups. The distribution of epitopes in larvae, particularly 3 h schistosomula, generally mirrored that in adults. A change in distribution of epitopes coincided with transformation from cercaria to schistosomulum providing a marker for this process. Two MABs reacted with surface membranes of intact cercariae, and 5 with the surfaces of intact 3 h schistosomula. The target epitopes of these 5 MABs were present in the tegument of adults, but not accessible externally. These observations suggest masking of antigens, not shedding, in the course of development. Indeed, no MAB reacted with intact lung or liver worms unless they were damaged. Three MABs reacted with membranes of the tegument and most other tissues, implying properties in common. Evidence for shared functions between epithelia was provided by the reactivity of 2 MABs with the tegument and protonephridia. Eight MABs bound both to the tegument and its cell bodies. Since the latter contains the machinery for protein synthesis, it is difficult to explain why 5 MABs bound to the tegument alone. Little cross-reactivity was found with the tissues of the tapeworm Hymenolepis, rather more with the closely related Schistosoma japonicum. It was concluded that tegument antigens are to a degree tissue specific but definitely not stage specific. Copyright © Cambridge University Press 1989 Schistosoma mansoni nationallicence epitopes nationallicence anti-tegument antibodies nationallicence antigen masking nationallicence tegument antigens nationallicence Riengrojpitak Suda Department of Biology, University of York, York YO1 5DD Vojvodic Maria Department of Biology, University of York, York YO1 5DD Boot C. Department of Biology, University of York, York YO1 5DD Wilson R. A. Department of Biology, University of York, York YO1 5DD Parasitology Cambridge University Press 98/2(1989-04), 213-225 0031-1820 98:2<213 1989 98 PAR https://doi.org/10.1017/S0031182000062120 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000062120 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Riengrojpitak Suda Department of Biology, University of York, York YO1 5DD NATIONALLICENCE 700 1- Vojvodic Maria Department of Biology, University of York, York YO1 5DD NATIONALLICENCE 700 1- Boot C. Department of Biology, University of York, York YO1 5DD NATIONALLICENCE 700 1- Wilson R. A. Department of Biology, University of York, York YO1 5DD NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/2(1989-04), 213-225 0031-1820 98:2<213 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge