caa a22 4500 386400164 CHVBK 20180307112155.0 cr unu---uuuuu 161130e198904 xx s 000 0 eng 10.1017/S003118200006217X doi S003118200006217X pii (NATIONALLICENCE)cambridge-10.1017/S003118200006217X Radiorespirometric detection of macrofilaricidal activity in vitro [Elektronische Daten] An in vitro method for studying radiorespiration has been adapted to single macrofilariae. Using this method viable (but not heat-killed) Dipetalonema viteae and Onchocera gibsoni macrofilariae evolved measurable amounts of 14CO2 from L-[U-14C]glutamine. Nonlinear and less uniform rates of 14CO2 evolution were demonstrated with D-[U-14C]glucose, [1-14C]acetate and [1-14C]octanoate. These findings led us to develop an in vitro screen in which inhibition of 14CO2 evolution from L-[U-14C]glutamine was used as a parameter for gauging macrofilaricidal activity. Using this screen we have examined the activity of 17 ‘so-called' antifilarial standards and found a greater degree of sensitivity than shown by other biochemical criteria. Other data obtained suggest a role for radiorespirometry in determining the viability of fragments of Onchocerca tissue. Copyright © Cambridge University Press 1989 radiorespirometry nationallicence Dipetalonema viteae nationallicence Onchocerca gibsoni nationallicence macrofilariae nationallicence Comley J. C. W. Department of Molecular Sciences, The Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS Rees M. J. Department of Molecular Sciences, The Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS Parasitology Cambridge University Press 98/2(1989-04), 259-264 0031-1820 98:2<259 1989 98 PAR https://doi.org/10.1017/S003118200006217X text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S003118200006217X text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Comley J. C. W. Department of Molecular Sciences, The Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS NATIONALLICENCE 700 1- Rees M. J. Department of Molecular Sciences, The Wellcome Research Laboratories, Langley Court, Beckenham, Kent BR3 3BS NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/2(1989-04), 259-264 0031-1820 98:2<259 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge