caa a22 4500 386400172 CHVBK 20180307112155.0 cr unu---uuuuu 161130e198904 xx s 000 0 eng 10.1017/S0031182000062168 doi S0031182000062168 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000062168 Role of hypochlorous acid in Trypanosoma musculi killing by phagocytes [Elektronische Daten] Trypanosoma musculi are readily killed when phagocytosed by mononuclear phagocytes but the nature of the mediators of this cytotoxicity is unclear. Among the most potent mediators are oxygen-derived species. The generation of chemilumine-scence (CL) by peritoneal macrophages from 12 day T. musculi-infected mice, which phagocytose and kill parasites when opsonizing antibodies are present, was recorded in the presence of antibody-coated trypanosomes. Taurine, a specific quencher of hypochlorous acid (HOCl) inhibited CL production by peritoneal macrophages, showing that HOCl is produced during phagocytosis of T. musculi. In vitro, HOCl alone exerted a powerful trypanocidal activity which was inhibited in the presence of specific quenchers. The role of HOCl generated by phagocytes in trypanosome killing was studied using granulocytes which produce more oxygen-derived species than macrophages when stimulated. Phorbol myristate acetate-triggered granulocytes can destroy T. musculi and trypanosome killing is inhibited in the presence of taurine. These data demonstrate that HOC1 produced by phagocytes can effectively destroy T. musculi. Copyright © Cambridge University Press 1989 hypochlorous acid nationallicence chemiluminescence nationallicence macrophage nationallicence Trypanosoma musculi nationallicence Vincendeau P. Laboratoire de Parasitologie, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France Daulouède S. Laboratoire de Parasitologie, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France Veyret B. Laboratoire de Chimie Physique A, Université de Bordeaux I, 33405 Talence Cedex, France Parasitology Cambridge University Press 98/2(1989-04), 253-257 0031-1820 98:2<253 1989 98 PAR https://doi.org/10.1017/S0031182000062168 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000062168 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Vincendeau P. Laboratoire de Parasitologie, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France NATIONALLICENCE 700 1- Daulouède S. Laboratoire de Parasitologie, Université de Bordeaux II, 146 rue Léo Saignat, 33076 Bordeaux Cedex, France NATIONALLICENCE 700 1- Veyret B. Laboratoire de Chimie Physique A, Université de Bordeaux I, 33405 Talence Cedex, France NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/2(1989-04), 253-257 0031-1820 98:2<253 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge