caa a22 4500 386400237 CHVBK 20180307112156.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S0031182000059023 doi S0031182000059023 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059023 An analysis of staining methods for the detection of Cryptosporidium spp. oocysts in water-related samples [Elektronische Daten] The ability of five staining techniques, originally developed for the rapid identification of Cryptosporidium spp. oocysts in faecal samples, to detect oocysts in water and water-related samples was assessed. All the stains used (modified Ziehl Neelsen, auramine-phenol (Lempert), Wright-Giemsa, safranin-methylene blue and FITC-labelled monoclonal antibody) stained oocysts after storage in water for 2 months at 4 °C (71-89 % of control values). Storage of oocysts below 0 °C greatly reduced the staining ability of auramine-phenol. With the exception of oocysts stored in raw and final waters, the histochemical stains proved less useful in detecting oocysts than the monoclonal antibody. Organisms of similar size and shape took up these stains, causing confusion in interpretation. Cold Ziehl Neelsen and the FITC-labelled monoclonal antibody were best at identifying oocysts from a waterborne outbreak. Screening with a fluorescent antibody, followed by confirmation with cold Ziehl Neelsen, where possible, are the currently recommended procedures for the detection of oocysts in water-related samples. Copyright © Cambridge University Press 1989 Cryptosporidium spp nationallicence detection nationallicence staining techniques nationallicence oocysts nationallicence Smith H. V. Scottish Parasite Diagnostic Laboratory, Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW McDiarmid A. Department of Civil Engineering and Environmental Health, University of Strathclyde, Glasgow DG1 1XN Smith A. L. Scottish Parasite Diagnostic Laboratory, Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW Hinson A. R. Scottish Parasite Diagnostic Laboratory, Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW Gilmour R. A. Department of Civil Engineering and Environmental Health, University of Strathclyde, Glasgow DG1 1XN Parasitology Cambridge University Press 99/3(1989-12), 323-327 0031-1820 99:3<323 1989 99 PAR https://doi.org/10.1017/S0031182000059023 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059023 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Smith H. V. Scottish Parasite Diagnostic Laboratory, Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW NATIONALLICENCE 700 1- McDiarmid A. Department of Civil Engineering and Environmental Health, University of Strathclyde, Glasgow DG1 1XN NATIONALLICENCE 700 1- Smith A. L. Scottish Parasite Diagnostic Laboratory, Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW NATIONALLICENCE 700 1- Hinson A. R. Scottish Parasite Diagnostic Laboratory, Department of Bacteriology, Stobhill General Hospital, Glasgow G21 3UW NATIONALLICENCE 700 1- Gilmour R. A. Department of Civil Engineering and Environmental Health, University of Strathclyde, Glasgow DG1 1XN NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/3(1989-12), 323-327 0031-1820 99:3<323 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge