caa a22 4500 386400253 CHVBK 20180307112156.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S0031182000058996 doi S0031182000058996 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000058996 Study on genetic polymorphism of Leishmania infantum through the analysis of restriction enzyme digestion patterns of kinetoplast DNA [Elektronische Daten] Twenty-nine Leishmania infantum strains characterized by different host source, tropism and belonging to 6 zymodemes, were examined by restriction enzyme analysis of kinetoplast DNA (kDNA) using 15 endonucleases. The enzymes which produced only one fragment revealed full identity between all the strains examined, while those producing many bands gave different electrophoretic patterns. They were interpreted with the aid of numerical analyses (cluster and multifactorial analysis). The results show a cline of genetic variability among the strains, the highest similarity being observed between most of the viscerotropic strains isolated from man, dog, black rat and sandflies. The strain agents of human cutaneous leishmaniasis show a varying degree of genetic divergence from this group, which appears more evident when characters from isoenzymes are considered. Copyright © Cambridge University Press 1989 Leishmania infantum nationallicence kinetoplast DNA nationallicence genetic polymorphism nationallicence isoenzymes nationallicence Angelici M. C. Laboratorio di Parassitologia, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Roma, Italy Gramiccia M. Laboratorio di Parassitologia, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Roma, Italy Gradoni L. Laboratorio di Parassitologia, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Roma, Italy Parasitology Cambridge University Press 99/3(1989-12), 301-309 0031-1820 99:3<301 1989 99 PAR https://doi.org/10.1017/S0031182000058996 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000058996 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Angelici M. C. Laboratorio di Parassitologia, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Roma, Italy NATIONALLICENCE 700 1- Gramiccia M. Laboratorio di Parassitologia, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Roma, Italy NATIONALLICENCE 700 1- Gradoni L. Laboratorio di Parassitologia, Istituto Superiore di Sanitá, viale Regina Elena 299, 00161 Roma, Italy NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/3(1989-12), 301-309 0031-1820 99:3<301 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge