caa a22 4500 38640030X CHVBK 20180307112156.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S003118200005900X doi S003118200005900X pii (NATIONALLICENCE)cambridge-10.1017/S003118200005900X Leishmania mexicana mexicana: quantitative analysis of the intracellular cycle [Elektronische Daten] The complete intracellular cycle of the Leishmania mexicana mexicana G. S. strain was quantified in human macrophages and in the mouse IC-21 macrophage line utilizing a culture system that allows the direct observation of individual intracellular parasites. A wide range of pre-replicative lag periods exists, implying that promastigotes may be in any phase of their DNA synthetic cycle when phagocytosed by the macrophage. Amastigotes replicated 2-3 times, after which the host cell died and liberated amastigotes that were taken up by other macrophages and continued to replicate. The mean amastigote population-doubling time in human macrophages (17.5 h) was not statistically different from promastigotes growing in axenic culture (16.4 h), but was nearly 2-fold less than amastigotes growing in mouse-derived IC-21 macrophages (33.7 h). These observations are markedly different from cover-glass culture assays of Leishmania-macrophage interactions and provide an unambiguous description of the intracellular cycle of Leishmania mexicana mexicana. Copyright © Cambridge University Press 1989 parasitic protozoa nationallicence Leishmania nationallicence intracellular cycle nationallicence macrophages nationallicence Doyle P. S. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA Engel J. C. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA Gam A. A. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA Dvorak J. A. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA Parasitology Cambridge University Press 99/3(1989-12), 311-316 0031-1820 99:3<311 1989 99 PAR https://doi.org/10.1017/S003118200005900X text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S003118200005900X text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Doyle P. S. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA NATIONALLICENCE 700 1- Engel J. C. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA NATIONALLICENCE 700 1- Gam A. A. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA NATIONALLICENCE 700 1- Dvorak J. A. Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/3(1989-12), 311-316 0031-1820 99:3<311 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge