caa a22 4500 386400334 CHVBK 20180307112156.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S0031182000059114 doi S0031182000059114 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059114 Gibson W. C. Department of Pathology, University of Bristol, School of Veterinary Science, Langford, Bristol BS18 7DU Analysis of a genetic cross between Trypanosoma brucei rhodesiense and T. b. brucei [Elektronische Daten] [W. C. Gibson] Two trypanosome clones, representing East and West African homozygotes at 2 isoenzyme loci (T. b. rhodesiense MHOM/ZM/74/58 [CLONE B] and T. b. brucei MSUS/CI/78/TSW 196 [CLONE A]), were cotransmitted through tsetse flies and the resulting trypanosome populations checked for the presence of non-parental karyotypes by pulsed-field gel electrophoresis. Ten clones isolated from these populations proved to have 5 different recombinant genotypes by analysis of nuclear and kinetoplast DNA (kDNA) polymorphisms. It is inferred that genetic exchange occurred between the 2 trypanosome clones in the fly, as previously reported for 2 other T. brucei spp. clones by Jenni and colleagues. For the most part, the hybrid clones shared many characteristics with both parents and their genotypes were consistent with segregation and reassortment of parental alleles. The least amount of genetic material exchanged was kDNA alone. Regarding the mechanism of genetic exchange, several hybrid clones had identical and unique nuclear DNA polymorphisms, but different kDNA type. Assuming that the same reassortment of nuclear markers is unlikely to occur by chance, these clones most probably arose from a predecessor carrying both types of kDNA. Copyright © Cambridge University Press 1989 Trypanosoma brucei rhodesiense nationallicence Trypanosoma brucei brucei nationallicence genetic exchange nationallicence tsetse nationallicence variant surface glycoprotein gene nationallicence Parasitology Cambridge University Press 99/3(1989-12), 391-402 0031-1820 99:3<391 1989 99 PAR https://doi.org/10.1017/S0031182000059114 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059114 text/html Onlinezugriff via DOI NATIONALLICENCE 100 1- Gibson W. C. Department of Pathology, University of Bristol, School of Veterinary Science, Langford, Bristol BS18 7DU NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/3(1989-12), 391-402 0031-1820 99:3<391 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge