caa a22 4500 386400377 CHVBK 20180307112156.0 cr unu---uuuuu 161130e198912 xx s 000 0 eng 10.1017/S0031182000059126 doi S0031182000059126 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000059126 Properties of phenol oxidase in Fasciola gigantica [Elektronische Daten] Phenol oxidase of Fasciola gigantica exists both as the soluble form as well as the membrane-bound form. The membrane-bound enzyme is considered to be a tyrosinase type because it is capable of oxidizing mono- and diphenol and is inefficient in oxidizing paraphenols. The soluble enzyme is a laccase type showing more affinity to various diphenols and paraphenols. Membrane-bound enzyme exists as isoenzymes, showing 3 fractions, of which the slow-moving fraction is capable of oxidizing both 4-methyl catechol and catechol, whereas the two remaining fractions are specific to 4-methyl catechol only. Soluble enzyme exists as a single homogeneous form showing affinity to both mono- and diphenols. Inhibition of the enzyme by potassium iodide and mercuric chloride indicates the active tyrosyl and SH groups of the enzyme. Inhibition of the enzyme by sodium diethyl dithiocarbamate and phenyl thiourea indicates that copper is the prosthetic group of the enzyme. Copyright © Cambridge University Press 1989 Fasciola gigantica nationallicence phenol oxidase nationallicence specificity nationallicence multiple form nationallicence egg-shell formation nationallicence tanning nationallicence Nellaiappan K. Department of Zoology, University of Madras, Guindy Campus, Madras 600 025, India Devasundari A. Fatima Department of Zoology, University of Madras, Guindy Campus, Madras 600 025, India Dhandayuthapani S. Department of Zoology, University of Madras, Guindy Campus, Madras 600 025, India Parasitology Cambridge University Press 99/3(1989-12), 403-407 0031-1820 99:3<403 1989 99 PAR https://doi.org/10.1017/S0031182000059126 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000059126 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nellaiappan K. Department of Zoology, University of Madras, Guindy Campus, Madras 600 025, India NATIONALLICENCE 700 1- Devasundari A. Fatima Department of Zoology, University of Madras, Guindy Campus, Madras 600 025, India NATIONALLICENCE 700 1- Dhandayuthapani S. Department of Zoology, University of Madras, Guindy Campus, Madras 600 025, India NATIONALLICENCE 773 0- Parasitology Cambridge University Press 99/3(1989-12), 403-407 0031-1820 99:3<403 1989 99 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge