caa a22 4500 386400431 CHVBK 20180307112156.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S0031182000061448 doi S0031182000061448 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061448 Transport processes of 2-deoxy-D-glucose in erythrocytes infected with Plasmodium yoelii, a rodent malaria parasite [Elektronische Daten] The transport processes of D-glucose in Plasmodium yoelii-infected mouse erythrocytes were investigated using 2-deoxy-D-glucose (2DOG), a non-metabolizable analogue of D-glucose. Infected cells showed an increase in the uptake of 2DOG compared to uninfected controls, and an effect which was more prominent in cells with mature-stage parasites. Kinetic studies measuring the initial rates of 2DOG uptake revealed two components in infected cells with late trophozoite and schizont-stage parasites: a simple diffusion system and a carrier (transporter)-mediated system. The transporter was common for D-glucose and 2DOG and had a kinetic constant indicating a high affinity for 2DOG (the Km = 0·18 mM and the Vmax = 0·61 mmol/1010 cells/min), as compared to the constant of the mouse erythrocyte carrier (the Km = 10 mM and the Vmax = 1·8 mmol/1010 cells/min). Determination of the distribution of [3H]2DOG in infected cells and experiments with metabolic inhibitors indicated that the simple diffusion system localizes in the membrane of host cells and the transporter in the parasite plasma membrane. The parasite glucose transporter was much less sensitive to cytochalasin B than that of the host cells and the uptake of 2DOG via the transporter was dependent on energy. Based on these findings, the following features emerge: D-glucose first gains access to the cytosol of infected erythrocytes via the simple diffusion system, which appears after infection by the parasite, and an active uptake against the concentration gradient takes place at the parasite plasma membrane via the parasite glucose transporter in an energy dependent manner. Finally, an energy transduction mechanism for the transport of glucose across the parasite plasma membrane is discussed. Copyright © Cambridge University Press 1989 Plasmodium yoelii nationallicence transport processes nationallicence 2-deoxy-D-glucose nationallicence glucose nationallicence erythrocytes nationallicence Izumo A. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan Tanabe K. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan Kato M. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan Doi S. Department of Legal Medicine, Kinki University School of Medicine, Osaka-Sayama, Osaka 589, Japan Maekawa K. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan Takada S. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan Parasitology Cambridge University Press 98/3(1989-06), 371-379 0031-1820 98:3<371 1989 98 PAR https://doi.org/10.1017/S0031182000061448 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061448 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Izumo A. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan NATIONALLICENCE 700 1- Tanabe K. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan NATIONALLICENCE 700 1- Kato M. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan NATIONALLICENCE 700 1- Doi S. Department of Legal Medicine, Kinki University School of Medicine, Osaka-Sayama, Osaka 589, Japan NATIONALLICENCE 700 1- Maekawa K. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan NATIONALLICENCE 700 1- Takada S. Department of Medical Zoology, Osaka City University Medical School, Asahi-machi, Abeno-ku, Osaka 545, Japan NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/3(1989-06), 371-379 0031-1820 98:3<371 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge