caa a22 4500 38640044X CHVBK 20180307112156.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S0031182000061503 doi S0031182000061503 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061503 Antibody to carbohydrate and polypeptide epitopes on the surface of schistosomula of Schistosoma mansoni in Egyptian patients with acute and chronic schistosomiasis [Elektronische Daten] 125I-Schistosoma mansoni schistosomulum surface antigens were immunoprecipitated with human antibodies from individual Egyptian patients diagnosed as being either acutely or chronically infected with S. mansoni. Both sets of patients were found to have IgG antibodies in their sera capable of immunoprecipitating the major Mr > 200, 38 and 32K antigens. However, the immunoprecipitation of the Mr 200K antigen was found to constitute a significantly greater proportion of the total precipitate achieved with acute sera than with chronic sera. The Mr 38 and 32K antigens were more variably precipitated by the acute sera than the chronic sera but the proportion of the total precipitation that these two antigens constituted was not found to be significantly different between the two sets of sera. Immunoprecipitation with pooled antibodies absorbed with egg and adult worm homogenates which had been treated to remove either carbohydrate or polypeptide epitopes demonstrated that the Mr > 200K antigen was the principal target of egg-cross-reactive anti-carbohydrate antibody amongst the antigens detected. The Mr 38 and 32K antigens were found to be precipitated by antibodies to protease-sensitive and periodate-insensitive polypeptide epitopes. These results are consistent with egg-cross-reactive anti-carbohydrate IgG antibody making a greater contribution to schistosomulum surface recognition in acute infection than in chronic infection. Indeed the presence of a higher level of egg-cross-reactive and anti-carbohydrate antibody directed against schistosomulum surface epitopes in an acute serum pool than in a chronic serum pool was confirmed by measurement of antibody binding to whole schistosomula. Copyright © Cambridge University Press 1989 Schistosoma mansoni nationallicence schistosomula nationallicence antigens nationallicence human antisera nationallicence carbohydrate epitopes nationallicence polypeptide epitopes nationallicence Omer Ali P. Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA Mansour M. Biochemistry Department, U.S. Naval Medical Research Unit No. 3, Cairo, Arab Republic of Egypt Woody J. N. Biochemistry Department, U.S. Naval Medical Research Unit No. 3, Cairo, Arab Republic of Egypt Smithers S. R. Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA Simpson A. J. G. Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA Parasitology Cambridge University Press 98/3(1989-06), 417-424 0031-1820 98:3<417 1989 98 PAR https://doi.org/10.1017/S0031182000061503 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061503 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Omer Ali P. Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 700 1- Mansour M. Biochemistry Department, U.S. Naval Medical Research Unit No. 3, Cairo, Arab Republic of Egypt NATIONALLICENCE 700 1- Woody J. N. Biochemistry Department, U.S. Naval Medical Research Unit No. 3, Cairo, Arab Republic of Egypt NATIONALLICENCE 700 1- Smithers S. R. Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 700 1- Simpson A. J. G. Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/3(1989-06), 417-424 0031-1820 98:3<417 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge