caa a22 4500 386400490 CHVBK 20180307112157.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S0031182000061436 doi S0031182000061436 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061436 In vitro cytoadherence of Plasmodium falciparum-infected erythrocytes to melanoma cells: factors affecting adhesion [Elektronische Daten] A reproducible and standardized assay for measuring the cytoadherence of knobby Plasmodium falciparum-infected red cells to amelanotic melanoma cells was developed. Adhesion was dependent on temperature, haematocrit, and parasitaemia. Addition of EDTA to the binding medium reduced adhesion. Removal of protease-sensitive molecules on the surface of the infected cell abolished cytoadherence, whereas removal of carbohydrate residues by treatment of cells with neuraminidase or galactosidase promoted adhesion. Calcium, magnesium, fibrinogen or fibronectin in the medium had no effect on adhesion nor was there any enhancement of adhesion by pre-loading infected cells with calcium. Serum was essential for good adhesion. Adhesion was species specific for target cells; human endothelial or amelanotic melanoma cells were suitable target cells whereas bovine cells were not. The amelanotic melanoma cell could be formalin-fixed and still retain its adhesion properties. The binding properties of formalin-fixed amelanotic melanoma cells were not identical to those of endothelial or unfixed target cells. Copyright © Cambridge University Press 1989 Plasmodium falciparum nationallicence cytoadherence nationallicence amelanotic melanoma cells nationallicence erythrocytes nationallicence Sherman I. W. Department of Biology, University of California, Riverside, California 92521 Valdez Esther Department of Biology, University of California, Riverside, California 92521 Parasitology Cambridge University Press 98/3(1989-06), 359-369 0031-1820 98:3<359 1989 98 PAR https://doi.org/10.1017/S0031182000061436 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061436 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sherman I. W. Department of Biology, University of California, Riverside, California 92521 NATIONALLICENCE 700 1- Valdez Esther Department of Biology, University of California, Riverside, California 92521 NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/3(1989-06), 359-369 0031-1820 98:3<359 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge