caa a22 4500 386400539 CHVBK 20180307112157.0 cr unu---uuuuu 161130e198906 xx s 000 0 eng 10.1017/S0031182000061497 doi S0031182000061497 pii (NATIONALLICENCE)cambridge-10.1017/S0031182000061497 Aerobic to anaerobic transition in the carbohydrate metabolism of Schistosoma mansoni cercariae during transformation in vitro [Elektronische Daten] Schistosoma mansoni cercariae in water were shown to possess a largely aerobic energy metabolism, the Krebs cycle being the main terminal of carbohydrate breakdown. A metabolic transition towards a more anaerobic breakdown of carbo-hydrate could be achieved by incubation conditions which also stimulated biological transformation. Incubation of cercariae in a simple salt medium containing 5 mM glucose induced such a metabolic transition: beside carbon dioxide large amounts of lactate and pyruvate were excreted. The results indicate that the production of pyruvate was coupled to electron transfer in the respiratory chain. Some aspects of this unusual pyruvate production are discussed. The observed change in the end-product pattern of carbohydrate breakdown is very rapid: most of the switch occurred within 2 h. Our results show that the metabolic transition was triggered by the biological transformation itself, or by the same event that induces the biological transformation. The metabolic and the biological changes proceeded synchronously. Copyright © Cambridge University Press 1989 Schisotoma mansoni nationallicence carbohydrate metabolism nationallicence cercariae nationallicence metabolic transition nationallicence Van Oordt B. E. P. Laboratory of Veterinary Biochemistry, Utrecht University, P.O. Box 80.176, 3508 TD Utrecht, The Netherlands Tielens A. G. M. Laboratory of Veterinary Biochemistry, Utrecht University, P.O. Box 80.176, 3508 TD Utrecht, The Netherlands Van Den Bergh S. G. Laboratory of Veterinary Biochemistry, Utrecht University, P.O. Box 80.176, 3508 TD Utrecht, The Netherlands Parasitology Cambridge University Press 98/3(1989-06), 409-415 0031-1820 98:3<409 1989 98 PAR https://doi.org/10.1017/S0031182000061497 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1017/S0031182000061497 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Van Oordt B. E. P. Laboratory of Veterinary Biochemistry, Utrecht University, P.O. Box 80.176, 3508 TD Utrecht, The Netherlands NATIONALLICENCE 700 1- Tielens A. G. M. Laboratory of Veterinary Biochemistry, Utrecht University, P.O. Box 80.176, 3508 TD Utrecht, The Netherlands NATIONALLICENCE 700 1- Van Den Bergh S. G. Laboratory of Veterinary Biochemistry, Utrecht University, P.O. Box 80.176, 3508 TD Utrecht, The Netherlands NATIONALLICENCE 773 0- Parasitology Cambridge University Press 98/3(1989-06), 409-415 0031-1820 98:3<409 1989 98 PAR CC0 http://creativecommons.org/publicdomain/zero/1.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-cambridge